Proposed new data file standard for flow cytometry, version FCS 3.0
Proposed new data file standard for flow cytometry, version FCS 3.0
复制标题
DOI：
10.1002/(sici)1097-0320(19970601)28:2
复制
发表时间：
1997-06-01
期刊：
影响因子：
--
通讯作者：
Murphy, RF
中科院分区：
文献类型：
--
作者：
Seamer, LC;Bagwell, CB;Murphy, RF
1984年，第一个流式细胞术数据文件格式被提议为流式细胞术标准1.0（FCS 1.0）。FCS 1.0提供了一种统一的文件格式，允许在一台计算机上获取的数据在运行各种操作系统的其他计算机上正确读取和解释。该标准于1990年进行了修改，并被分析细胞学学会采用为FCS 2.0。在这里，我们报告FCS 2.0标准的更新，我们建议将其命名为FCS 3.0。我们保留了早期版本的基本四段结构（HEADER、TEXT、DATA和ANALYSIS），以尽可能保持分析软件的兼容性。本提案中描述的更改包括收集大于100兆字节的薯条的方法（在标准的早期版本中不可能），在文件的文本部分中包含国际字符，使用16位循环冗余校验验证数据完整性的方法，以及增加对聚类分析和时间采集的关键字顺序支持。本报告总结了ISAC数据文件标准委员会的工作。完整和详细的FCS 3.0标准可通过ISAC办公室获得[舍伍德集团，60 Revere Drive，Ste 500，Northbrook，IL 60062，电话：（847）480-9080 ext. 231，传真：（847）480-9282，电子邮件：isac@sherwood-group.com]或通过互联网访问ISAC万维网网站http://nucleus.immunol.washingtsn.edu/ISAC.html。(C)1997 Wiley-Liss，Inc.
In 1984, the first flow cytometry data file format was proposed as Flow Cytometry Standard 1.0 (FCS1.0). FCS 1.0 provided a uniform file format allowing data acquired on one computer to be correctly read and interpreted on other computers running a variety of operating systems. That standard was modified in 1990 and adopted by the Society of Analytical Cytology as FCS 2.0. Here, we report on an update of the FCS 2.0 standard which we propose to designate FCS 3.0. We have retained the basic four segment structure of earlier versions (HEADER, TEXT, DATA and ANALYSIS) in order to maintain analysis software compatibility, where possible. The changes described in this proposal include a method to collect fries larger than 100 megabytes (not possible in earlier versions of the standard), the Inclusion of international characters in the TEXT portions of the file, a method of verifying data integrity using a 16-bit cyclic redundancy check, and increased keyword ord support far cluster analysis and time acquisition. This report summarizes the work of the ISAC Data File Standards Committee. The complete and detailed FCS 3.0 standard is available through the ISAC office [Sherwood Group, 60 Revere Drive, Ste 500, Northbrook, IL 60062, phone: (847) 480-9080 ext. 231, fax: (847) 480-9282, E-mail: isac@sherwood-group.com] or through the internet at the ISAC WWW site, http://nucleus.immunol.washingtsn.edu/ISAC.html. (C) 1997 Wiley-Liss, Inc.