The complete coding sequence of hepatitis C virus genotype 5a, the predominant genotype in South Africa
The complete coding sequence of hepatitis C virus genotype 5a, the predominant genotype in South Africa
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DOI：
10.1006/bbrc.1997.6902
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发表时间：
1997-07-09
影响因子：
3.1
通讯作者：
Elliott, RM
中科院分区：
文献类型：
--
作者：
Chamberlain, RW;Adams, NJ;Elliott, RM
丙型肝炎病毒（HCV）基因型5a是南部非洲的主要基因型，在来自南非地区的受感染献血者中具有高流行率。我们已经确定了相应的核苷酸序列的完整编码区的HCV分离株，EUH 1480，以前被归类为基因型5a，从爱丁堡血友病。该序列含有一个单一的开放阅读框（ORF）编码的3014个氨基酸的多聚蛋白。与其他HCB基因型的多蛋白序列（ORF从3008到3037个氨基酸不等）进行比较，表明观察到的大小变化是由于包膜2和非结构5A蛋白长度的差异造成的。HCV基因型5与基因型1c的核苷酸差异（24.9%的氨基酸差异）为29.4%，与基因型2a的核苷酸差异（30.3%的氨基酸差异）为32.5%。可用的全长核苷酸序列的系统发育分析显示EUH 1480形成不同于其他HCV类型的分支，证实了5a型作为单独基因型的分类。(C)北京：科学出版社.
Hepatitis C virus (HCV) genotype 5a is the predominant genotype in southern Africa with a high prevalence amongst infected blood donors from areas in South Africa. We have determined the nucleotide sequence corresponding to the complete coding region of an HCV isolate, EUH1480, previously classified as genotype 5a, from and Edinburgh haemophiliac. The sequence contained a single open reading frame (ORF) coding for a polyprotein of 3014 amino acids. Comparison with the polyprotein sequences from other HCB genotypes, where the ORF varies from 3008 to 3037 amino acids, showed the observed variation in size was due to differences in lengths of the envelope 2 and the nonstructural 5A proteins. The sequence divergence of HCV genotype 5 ranged from 29.4% nucleotide differences (24.9% amino acid differences) compared with genotype 1c to 32.5% nucleotide differences (30.3% amino acid differences) compared with 2a. Phylogenetic analysis of the available full length nucleotide sequences showed EUH1480 to form a branch distinct from the other HCV types, confirming the classification of type 5a as a separate genotype. (C) 1997 Academic Press.