Overexpression of a Miscanthus sacchariflorus yellow stripe-like transporter MsYSL1 enhances resistance of Arabidopsis to cadmium by mediating metal ion reallocation
Overexpression of a Miscanthus sacchariflorus yellow stripe-like transporter MsYSL1 enhances resistance of Arabidopsis to cadmium by mediating metal ion reallocation
复制标题
芒草黄色条纹状转运蛋白MsYSL1的过度表达通过介导金属离子重新分配增强拟南芥对镉的抗性
DOI：
10.1007/s10725-018-0376-6
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发表时间：
2018-05-01
影响因子：
4.2
通讯作者：
Jiang, Dean
中科院分区：
文献类型：
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作者：
Chen, Houming;Zhang, Cheng;Jiang, Dean
黄条样转运蛋白（YSL）家族通过转运与植物铁载体或烟酰胺（NA）螯合的金属离子来介导体内多种矿物元素的摄取、转运和分布。然而，对植物中YSL基因对镉的作用知之甚少。在这项研究中，我们首先克隆和表征的YSL基因家族的重要成员，MsYSL 1，从生物能源植物芒草sacchariflorus。MsYSL 1定位于质膜，在整个幼苗中广泛表达，在茎中表达量最高。此外，其在根中的表达被过量的锰（Mn）、镉（Cd）和铅以及铁（Fe）、锌（Zn）和铜的缺乏所刺激。在酵母中的功能互补表明MsYSL 1对Fe（II）-NA和Zn-NA有转运活性，但对Cd-NA无转运活性。虽然他们表现出与野生型在正常培养条件下没有显着差异，MsYSL 1过表达的拟南芥品系表现出更高的抗镉伴随着较长的根长，较低的镉，锌，锰的水平在根，镉，铁，锰的易位率较高的镉胁迫。此外，与NA合成，金属转运，远距离运输，镉排斥相关的基因在镉胁迫下的转基因株系的诱导。因此，MsYSL 1可能是多种金属NAs参与镉解毒的重要转运蛋白，通过介导其他金属离子的重新分配而发挥作用。
The yellow stripe-like (YSL) family of transporters mediates the uptake, translocation, and distribution of various mineral elements in vivo by transferring metal ions chelated with phytosiderophore or nicotianamine (NA). However, little is known about the roles of the YSL genes against cadmium in planta. In this study, we first cloned and characterized a vital member of the YSL gene family, MsYSL1, from the bioenergy plant Miscanthus sacchariflorus. MsYSL1 localized in the plasma membrane and was widely expressed throughout the whole seedling with the highest expression level in the stem. In addition, its expression in the root was stimulated by excess manganese (Mn), cadmium (Cd), and lead, and a shortage of iron (Fe), zinc (Zn), and copper. Functional complementation in yeast indicated that MsYSL1 showed transport activity for Fe(II)–NA and Zn–NA, but not for Cd–NA. Although they exhibited no significant differences versus the wild type under normal cultivation conditions, MsYSL1-overexpressing Arabidopsis lines displayed a higher resistance to Cd accompanied by longer root lengths, lower Cd, Zn, and Mn levels in roots, and higher Cd, Fe, and Mn translocation ratios under Cd stress. Moreover, genes related to NA synthesis, metal translocation, long-distance transport, and Cd exclusion were highly induced in transgenic lines under Cd stress. Thus, MsYSL1 may be an essential transporter for diverse metal–NAs to participate in the Cd detoxification by mediating the reallocation of other metal ions.