Novel planar glucose biosensors for continuous monitoring use
Novel planar glucose biosensors for continuous monitoring use
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DOI：
10.1016/j.bios.2004.09.010
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发表时间：
2005-04-15
影响因子：
12.6
通讯作者：
Messeri, D
中科院分区：
文献类型：
--
作者：
Ricci, F;Moscone, D;Messeri, D
用于连续监测的新型平面葡萄糖生物传感器已经被开发出来。该电极采用“丝网印刷”技术生产，重现性好，成本低，具有大规模生产的可能性。在酶固定化之前，电极要用铁氰化铁(普鲁士蓝)进行化学修饰。这使得能够在较低的施加电位(约0.0V对银/氯化银)下检测由GOD催化的酶反应产生的过氧化氢，从而极大地限制任何电化学干扰。普鲁士蓝(PB)层在工作条件下(pH 7.4)表现出很高的稳定性，并且在RT下干燥储存1年后，没有观察到活性损失。组装的葡萄糖生物传感器对葡萄糖具有很高的灵敏度，并具有长期的操作和储存稳定性。在连续流动系统中，在所有分析参数优化后，葡萄糖生物传感器可以检测到低至0.025 mm的葡萄糖浓度，线性范围高达1.0mM。这些探头还在连续流动模式下进行了50-60 h的测试，以评估其操作稳定性。将浓度为0.5 mM的葡萄糖连续通入生物传感器壁喷射池，并连续监测过氧化氢还原产生的电流。在50-60小时后，观察到的信号漂移约为30%。由于它们的高稳定性，这些传感器建议将这种生物传感器与微透析探头结合使用，用于临床上连续监测血糖。(C)2004爱思唯尔B.V.保留所有权利。
Novel planar glucose biosensors to be used for continuous monitoring have been developed. The electrodes are produced with the "screen printing" technique, and present a high degree of reproducibility together with a low cost and the possibility of mass production. Prior to enzyme immobilisation, electrodes are chemically modified with ferric hexacyanoferrate, (Prussian Blue). This allows the detection of the hydrogen peroxide produced by the enzymatic reaction catalysed by GOD, at low applied potential (ca. 0.0 V versus Ag/AgCl), highly limiting any electrochemical interferences. The layer of Prussian Blue (PB) showed a high stability at the working conditions (pH 7.4) and also after 1 year of storage dry at RT, no loss of activity was observed. The assembled glucose biosensors, showed high sensitivity towards glucose together with a long-term operational and storage stability. In a continuous flow system, with all the analytical parameters optimised, the glucose biosensors detected glucose concentration as low as 0.025 mM with a linear range up to 1.0 mM.These probes were also tested over 50-60 h in a continuous flow mode to evaluate their operational stability. A 0.5 mM concentration of glucose was continuously fluxed into a biosensor wall-jet cell and the current due to the hydrogen peroxide reduction was continuously monitored. After 50-60 h, the drift of the signal observed was around 30%.Because of their high stability, these sensors suggest the possibility of using such biosensors, in conjunction with a microdialysis probe, for a continuous monitoring of glucose for clinical purposes. (c) 2004 Elsevier B.V. All rights reserved.