Tyrosyl-base-phenylalanyl intercalation in gene 5 protein-DNA complexes: proton nuclear magnetic resonance of selectively deuterated gene 5 protein.
Tyrosyl-base-phenylalanyl intercalation in gene 5 protein-DNA complexes: proton nuclear magnetic resonance of selectively deuterated gene 5 protein.
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基因 5 蛋白-DNA 复合物中的酪氨酰基-苯丙氨酰嵌入：选择性氘化基因 5 蛋白的质子核磁共振。
DOI：
10.1021/bi00616a028
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发表时间：
1978
期刊：
影响因子：
2.9
通讯作者：
I. Armitage
中科院分区：
文献类型：
--
作者：
J. Coleman;I. Armitage
d(pA)8 和 d(pT)8 复合物中寡脱氧核苷酸与基因 5 蛋白芳香族残基的相互作用已通过蛋白质的 1 H NMR 确定，其中五个酪氨酰残基已在 2,6 或 3,5 位选择性氘化。只有三个表面酪氨酰（26、41 和 56）的 3,5 质子与碱基相互作用。在配合物形成过程中经历碱基依赖性高场环流位移的其余芳香质子是苯丙氨酰质子，根据模型构建分配给 Phe(13)。间氟酪氨酰标记的蛋白质与 d(pT)4 和 d(pA)8 的复合物的 19F NMR 证实了三个表面酪氨酰的 3,5-酪氨酰位置处存在核环电流扰动。 19F(1H) 核 Overhauser 效应的差异表达证实了两个埋藏酪氨酰残基和三个表面酪氨酰残基的存在。提出了一种涉及 Tyr(26)-碱基-Phe(13) 嵌入的 DNA 结合沟新模型。
The interactions of oligodeoxynucleotides with the aromatic residues of gene 5 protein in complexes with d(pA)8 and d(pT)8 have been determined by 1H NMR of the protein in which the five tyrosyl residues have been selectively deuterated either in the 2,6 or the 3,5 positions. Only the 3,5 protons of the three surface tyrosyls (26, 41, and 56) interact with the bases. The remainder of the aromatic protons undergoing base-dependent upfield ring-current shifts on complex formation are phenylalanyl protons, assigned to Phe(13) on the basis of model building. 19F NMR of the complexes of the m-fluorotyrosyl-labeled protein with d(pT)4 and d(pA)8 confirms the presence of ring-current perturbations of nuclei at the 3,5-tyrosyl positions of the three surface tyrosyls. Differential expression of the 19F(1H) nuclear Overhauser effect confirms the presence of two buried and three surface tyrosyl residues. A new model of the DNA binding groove is presented involving Tyr(26)-base-Phe(13) intercalation.