Blood markers of osteoclastic differentiation in periparturient dairy cows at different parities, with and without milk fever
Blood markers of osteoclastic differentiation in periparturient dairy cows at different parities, with and without milk fever
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DOI：
10.1016/j.rvsc.2020.05.009
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发表时间：
2020-08-01
影响因子：
2.4
通讯作者：
Yamagishi, Norio
中科院分区：
文献类型：
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作者：
Hatate, Kaoru;Kawashima, Chiho;Yamagishi, Norio
骨保护素 (OPG) 抑制破骨细胞 (OC) 分化。 OCs分泌的TRAP5b（抗酒石酸酸性磷酸酶5b）反映了成熟OCs的数量。本研究评估了不同胎次的奶牛和患有乳热 (MF) 的奶牛在分娩前后的这些 OC 相关标记。在 49 头荷斯坦弗里斯兰奶牛的第一胎 (n = 8)、第二胎 (n = 17)、第三胎 (n = 12) 和第四胎或更高胎次 (n = 12) 的产犊前 3 周 (-21 天) 和产后几天内测量血液 OPG 和 TRAP5b 浓度以及 OPG 与 TRAP5b (O/T) 的比率。还计算了 -21 天时的 O/T 与产犊时的 O/T (PreCOT) 之比。在第三胎及以上胎次中，有 7 头奶牛出现 MF（非 MF，n = 17）。无论 MF 是否发生，只有进入第二泌乳期的奶牛在妊娠最后一周血清 OPG 开始下降，而所有奶牛在产犊时血液 TRAP5b 均升高。仅在经产奶牛临产时 O/T 下降。 MF 奶牛在崩落时 O/T 的下降不太明显。仅在经产奶牛中，PreCOT 与泌乳数呈负相关（n = 41，rho = -0.50，P < .01）。这项研究表明，早产奶牛的 OC 向产犊分化逐渐减弱，这些指标可预测 MF 的风险。
Osteoprotegerin (OPG) inhibits osteoclast (OC) differentiation. TRAP5b (tartrate-resistant acid phosphatase 5b) secreted by OCs reflects the numbers of mature OCs. This study assessed these OC-related markers around parturition in cows of different parities and in cows with milk fever (MF). The blood OPG and TRAP5b concentrations, as well as the ratio of OPG to TRAP5b (O/T), were measured beginning 3 weeks before (-21 d) and over a few days after calving in 49 Holstein Friesian cows at first (n = 8), second (n = 17), third (n = 12), and fourth or greater (n = 12) parities. The ratio of O/T at -21 d to O/T at calving (PreCOT) was also calculated. In the third and greater parities, seven cows developed MF (non-MF, n = 17). Regardless of the development of MF, the serum OPG started to decline in the last week of gestation only in the cows entering the second lactation, while the blood TRAP5b increased at calving in all cows. O/T decreased toward parturition only in multiparous cows. The decrease in O/T at caving was less pronounced in MF cows. PreCOT was negatively correlated with lactation number only in multiparous cows (n = 41, rho = -0.50, P < .01). This study implied that OC differentiation toward calving was tapered in cows with advanced parities, and these indexes predict the risk of MF.