First molecular identification of Trypanosoma evansi from cattle in Syria
First molecular identification of Trypanosoma evansi from cattle in Syria
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发表时间：
2020-05
影响因子：
0.4
通讯作者：
Megasari Marsela;K. Hayashida;Alaa Terkawi;X. Xuan;C. Sugimoto;J. Yamagishi
中科院分区：
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作者：
Megasari Marsela;K. Hayashida;Alaa Terkawi;X. Xuan;C. Sugimoto;J. Yamagishi
伊氏锥虫是一种血液原生动物寄生虫，可在无限的地理区域内感染多种哺乳动物。它导致贫血，体重减轻，甚至死亡的感染牲畜，严重影响畜牧业。然而，完整的流行病学资料，T。伊文思缺乏，特别是在发展中国家，这种疾病的风险在很大程度上被低估。在这项研究中，2010年5月从叙利亚中部地区的荷斯坦黑白花杂交牛中收集的207份血液DNA样本进行了T。evansi，旨在确定寄生虫的流行程度。T.通过靶向内部转录间隔区（ITS）1区域的PCR筛选伊氏病毒，并且在207个样品中发现27个样品为阳性（13%），考虑到没有观察到临床症状，这是相对较高的。ITS 1扩增子随后进行RoTat 1.2-PCR以检测T.伊氏A型这是首次报道T.伊凡西在叙利亚。我们的研究表明，在叙利亚有必要对牛和其他家畜进行深入调查。
Trypanosoma evansi, the “surra” disease-causing agent, is a blood protozoan parasite that infects a wide range of mammalian species within an unlimited geographical region. It causes anemia, weight loss, and even death of the infected livestock that heavily affect animal husbandry. However, the full epidemiological information of T. evansi is lacking, especially in developing countries, and the risk of the disease is largely underestimated. In this study, 207 samples of blood DNA collected from Holstein Friesian crossbred cattle in the central region of Syria in May 2010 were screened for T. evansi, aiming to determine the prevalence of the parasite. T. evansi was screened by PCR targeting the internal transcribed spacer (ITS) 1 region, and 27 samples were found positive out of 207 (13%), which is relatively high considering that no clinical symptoms were observed. The ITS1 amplicons were later subjected to RoTat1.2-PCR for detection of T. evansi type A. This is the first report of molecular detection of T. evansi in Syria. Our study suggests that advanced investigations in cattle and other domestic animals are necessary in Syria.