Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions.
Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions.
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DOI：
10.2334/josnusd.49.133
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发表时间：
2007-06-01
影响因子：
1.9
通讯作者：
Waseem, Ahmad
中科院分区：
文献类型：
--
作者：
Kose, Osman;Lalli, Anand;Waseem, Ahmad
尽管干细胞在口腔上皮的稳态中起着关键作用，但该细胞群在组织中的位置是不确定的。疾病如何在体内影响这些细胞还有待阐明。在这项研究中，我们使用了六个分子标记，以确定干细胞在正常和患病的颊粘膜。对正常口腔粘膜（NOM）、角化过度（OHK）和口腔扁平苔藓（OLP）样本进行α 6和β 1整联蛋白、角蛋白15（K15）、黑素瘤相关硫酸软骨素蛋白聚糖（MCSP）、NG2（人类MCSP的大鼠同源物）和notch 1免疫染色。K15、NG2和beta1染色在NOM基底层连续，而alpha6和MCSP仅限于结缔组织乳头顶端的基底细胞。K15在OLP中下调，而α 6、β 1和MCSP在OLP和OHK中均上调。NG 2保持不变，所有样品中均不存在缺口1。因此，OLP和OHK中的干细胞表型可能响应于病理信号而改变。这些变化的分类是必不可少的，以了解成人干细胞在口腔疾病的发病机制中的作用，其特征在于异常角质形成细胞增殖和分化。
Despite the pivotal role of stem cells in homeostasis of oral epithelia the location of this cell population within the tissue is uncertain. How disease influences these cells in vivo also remains to be elucidated. In this study we have used six molecular markers to identify stem cells in normal and diseased buccal mucosa. Samples of normal oral mucosa (NOM), hyperkeratosis (OHK) and oral lichen planus (OLP) were immunostained for alpha6 and beta1 integrins, keratin 15 (K15), melanoma-associated chondroitin sulphate proteoglycan (MCSP), NG2 the rat homologue of human MCSP and notch 1. K15, NG2 and beta1 staining was continuous in the basal layer of NOM whilst alpha6 and MCSP were limited to basal cells at the tips of connective tissue papillae. K15 was downregulated in OLP whereas alpha6, beta1 and MCSP were upregulated in both OLP and OHK. NG2 remained unchanged and notch 1 was absent in all samples. Therefore, the stem cell phenotype in OLP and OHK maybe altered in response to pathological signaling. Classification of these changes is essential to understand the role of adult stem cells in the pathogenesis of oral diseases characterised by abnormal keratinocyte proliferation and differentiation.