Protein Phosphatase Type 1-Interacting Protein Ysw1 Is Involved in Proper Septin Organization and Prospore Membrane Formation during Sporulation
Protein Phosphatase Type 1-Interacting Protein Ysw1 Is Involved in Proper Septin Organization and Prospore Membrane Formation during Sporulation
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DOI：
10.1128/ec.00095-09
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发表时间：
2009-07-01
期刊：
影响因子：
--
通讯作者：
Tachikawa, Hiroyuki
中科院分区：
文献类型：
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作者：
Ishihara, Makoto;Suda, Yasuyuki;Tachikawa, Hiroyuki
酿酒酵母的孢子形成是一个发育过程，其中在二倍体细胞内产生四个单倍体孢子。Gip 1是蛋白磷酸酶1型的孢子形成特异性靶向亚基，其催化亚基Glc 7与隔蛋白沿着延伸的原孢子膜共定位，并且是隔蛋白组织和孢子壁形成所需的。然而，Gip 1-Glc 7磷酸酶促进这些事件的机制尚不清楚。在这里，我们表明，Ysw 1，孢子形成特异性卷曲螺旋蛋白，具有功能的关系Gip 1-Glc 7磷酸酶。YSW 1的过表达部分抑制gip 1的温度敏感等位基因的孢子形成缺陷。Ysw 1在双杂交试验中与Gip 1相互作用，这种相互作用是抑制所必需的。在孢子形成过程中，用绿色荧光蛋白标记的Ysw 1与septins和Gip 1沿着延伸的前孢子膜共定位。在ysw 1 Delta突变体中，孢子形成是部分缺陷的，细胞学分析显示，在ysw 1 Delta细胞中，隔蛋白结构受到干扰，原孢子膜延伸异常。这些结果表明，Ysw 1功能与Gip 1-Glc 7磷酸酶，以促进适当的隔蛋白组织和原孢子膜的形成。
Sporulation of Saccharomyces cerevisiae is a developmental process in which four haploid spores are generated inside a diploid cell. Gip1, a sporulation-specific targeting subunit of protein phosphatase type 1, together with its catalytic subunit, Glc7, colocalizes with septins along the extending prospore membrane and is required for septin organization and spore wall formation. However, the mechanism by which Gip1-Glc7 phosphatase promotes these events is unclear. We show here that Ysw1, a sporulation-specific coiled-coil protein, has a functional relationship to Gip1-Glc7 phosphatase. Overexpression of YSW1 partially suppresses the sporulation defect of a temperature-sensitive allele of gip1. Ysw1 interacts with Gip1 in a two-hybrid assay, and this interaction is required for suppression. Ysw1 tagged with green fluorescent protein colocalizes with septins and Gip1 along the extending prospore membrane during spore formation. Sporulation is partially defective in ysw1 Delta mutant, and cytological analysis revealed that septin structures are perturbed and prospore membrane extension is aberrant in ysw1 Delta cells. These results suggest that Ysw1 functions with the Gip1-Glc7 phosphatase to promote proper septin organization and prospore membrane formation.