Concentration-dependent dual action of locally applied N-methyl-D-aspartate on extracellular dopamine in the rat prefrontal cortex in vivo
Concentration-dependent dual action of locally applied N-methyl-D-aspartate on extracellular dopamine in the rat prefrontal cortex in vivo
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DOI：
10.1016/0304-3940(95)12164-1
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发表时间：
1995-12-08
影响因子：
2.5
通讯作者：
Botterblom, MHA
中科院分区：
文献类型：
--
作者：
Feenstra, MGP;vanderWeij, W;Botterblom, MHA
采用微透析法，将谷氨酸激动剂N-甲基-D-天冬氨酸(NMDA)通过自由活动大鼠的内侧前额叶皮质灌流20min，观察其对细胞外多巴胺(DA)浓度的影响。NMDA(1 MM)使DA增加到170-1500%，这取决于诱发的阵挛前肢抽动和抽搐的强度和持续时间。然而，NMDA(0.1 mM)使DA减少到61%。两种浓度的NMDA作用后，DA的代谢产物均减少。0.5 mM的竞争性NMDA拮抗剂D-AP-5可阻断0.1 mM和1 mM NMDA的作用。NMDA引起的释放和代谢减少可能是通过抑制性局部中间神经元或多突触回路的间接作用所致。
Using microdialysis, the glutamate agonist N-methyl-D-aspartate (NMDA) was perfused for 20 min through the medial prefrontal cortex of freely moving rats, and its effects on extracellular concentrations of dopamine (DA) were determined. NMDA (1 mM) increased DA to 170-1500%, depending on the intensity and duration of the clonic forelimb jerks and convulsions that were induced. NMDA (0.1 mM), however, decreased DA to 61%. Metabolites of DA were decreased after both concentrations of NMDA. The effects of both 0.1 mM and 1 mM NMDA were blocked by 0.5 mM of the competitive NMDA-antagonist D-AP-5. The NMDA-induced decrease in release and metabolism possibly results from an indirect action via an inhibitory local interneuron or polysynaptic circuit.