Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection against Aeromonas hydrophila
Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection against Aeromonas hydrophila
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DOI：
10.1016/j.fsi.2008.08.015
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发表时间：
2009-01-01
影响因子：
4.7
通讯作者：
Jeney, G.
中科院分区：
文献类型：
--
作者：
Yin, Guojun;Ardo, L.;Jeney, G.
研究了黄芪和灵芝对鲤鱼免疫功能的影响。试验用黄芪（0.5%）、灵芝（0.5%）和黄芪与灵芝的混合饲料（0.5%）饲养鱼5周。其他组的鱼接种疫苗（i. p.）针对嗜水气单胞菌/杀鲑气单胞菌（Shering Plough，埃塞克斯，英国）并饲喂与上述相同的饮食。对照鱼（阴性对照）和仅接种疫苗的鱼（阳性对照）饲喂不含草药补充剂的基础饮食。测定呼吸爆发活性、吞噬功能、溶菌酶活性和血浆循环抗体滴度。饲养5周后，用A.记录了死亡率和死亡率。结果表明，黄芪和灵芝配合投喂免疫鲤鱼和未免疫鲤鱼均能提高免疫鲤鱼的呼吸爆发活性、血液吞噬细胞和溶菌酶的吞噬能力以及血浆循环抗体滴度。鱼挑战A。阿瓜的存活率各不相同。最好的存活率（60%）是在用两种草药喂养的疫苗接种组中。而几乎90%的对照鱼（阴性对照）和60%的仅接种疫苗的鱼（阳性对照）死亡。(C)2008爱思唯尔有限公司保留所有权利。
The effect of Chinese herbs (Astragalus radix and Ganoderma lucidum) on immune response of carp was investigated. Fish were fed diets containing Astragalus (0.5%), Ganoderma (0.5%) and combination of two herbs (Astragalus 0.5% and Ganoderma 0.5%) for 5 weeks. Other groups of fish were vaccinated (i.p.) against Aeromonas hydrophila/Aeromonas salmonicida (Shering Plough, Essex, U.K.) at the beginning of the experiment and fed the same diets as described above. Control fish (negative control) and fish vaccinated only (positive control) were fed basal diets without supplements of herbs. The respiratory burst activity, phagocytosis, lysozyme activity and circulatory antibody titres in plasma were monitored. Following 5 weeks after feeding, fish were infected with A. hydrophila and mortalities were recorded. The results of this study showed that feeding non-vaccinated and vaccinated carp with combination of Astragalus and Ganoderma stimulated respiratory burst activity, phagocytosis of phagocytic cells in blood and lysozyme and circulatory antibody titres in plasma in vaccinated carp. Fish challenged with A. hydrophila had variable survival. The best survival (60%) was in vaccinated group fed with both herbs. while almost 90% of control fish (negative control) and 60% of fish vaccinated only (positive control) died. (C) 2008 Elsevier Ltd. All rights reserved.