Microelectrode measurements of low frequency electric field effects in cells and tissues.
Microelectrode measurements of low frequency electric field effects in cells and tissues.
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DOI：
10.1002/bem.2250130716
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发表时间：
1992
影响因子：
1.9
通讯作者：
K. McLeod
中科院分区：
文献类型：
--
作者：
K. McLeod
如果组织的形态和电导率已知，则可以计算诱导到组织中的电场的平均强度，并且这些值提供给定电场暴露水平的剂量估计。然而，活体组织的微观解剖结构，包括间隙连接、紧密连接、高电荷的细胞外壳和细胞外基质，以及复杂的细胞形状，阻碍了对电场和电流分布的详细描述，这些分布在实际响应电场的细胞附近。这表明，更有用的电场剂量度量可能是基于对细胞的诱导物理效应。电场对细胞至少有三种不同的物理效应：正常的质膜电位将被改变；细胞外表面的离子电流和离子分布将被改变；机械力会施加在细胞表面。原则上，每一种效应都可以通过应用特定的微电极技术来测量。本文讨论了使用各种细胞内和细胞外记录方法来获得剂量学值的可行性，以及这些测量方法对我们理解电场与生物组织相互作用的贡献。
The average intensities of electric fields induced into tissue can be calculated if the morphology and conductivities of the tissue are known, and such values provide one estimate of dosage for a given field exposure level. However, the microanatomical structures of living tissue, which include gap junctions, tight junctions, highly charged cell coats, and extracellular matrices, as well as complex cell shapes, precludes a detailed characterization of the field and current distribution near the cells which are actually responding to the electric fields. This suggests that a more useful electric field dose metric may be one based on an induced physical effect on the cells. Electric fields have at least three distinct physical effects on cells: the normal plasma membrane potential will be altered; the ionic currents and ion distributions at the extracellular surface will be modified; and mechanical forces will be imposed at the cell surface. Each of these effects can, in principle, be measured through the application of specific microelectrode techniques. Here, the feasibility of using various intracellular and extracellular recording methods to obtain dosimetric values, as well as the contribution these measurements could make to our understanding of electric field interactions with biological tissue, are discussed.