Expressed sequence tags (ESTs) from the salivary glands of the tick Amblyomma cajennense (Acari: Ixodidae)
Expressed sequence tags (ESTs) from the salivary glands of the tick Amblyomma cajennense (Acari: Ixodidae)
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DOI：
10.1016/j.toxicon.2007.12.011
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发表时间：
2008-04-01
期刊：
影响因子：
2.8
通讯作者：
Junqueira-de-Azevedo, Inacio L. M.
中科院分区：
文献类型：
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作者：
Batista, Isabel F. C.;Chudzinski-Tavassi, Ana M.;Junqueira-de-Azevedo, Inacio L. M.
新热带蜱Amblyomma cajennense是家畜的重要害虫，是南美洲最常见的叮咬人类的蜱虫，也是巴西斑点热（由立克次氏体引起）的主要媒介，这是一种致命的人类疾病。本研究的目的是描述A. cajennense唾液腺转录组表达序列标签（EST）。我们报告的1754个克隆从cDNA文库，这揭示了主要涉及的止血过程中的蛋白质，特别是蛋白酶及其抑制剂的转录本的分析。值得注意的是，发现了五种可能的丝氨酸蛋白酶抑制剂，包括一种具有独特结构的分子，该分子含有水蛭素抑制剂活性基序的重复序列。此外，还描述了可能对宿主免疫系统有活性或作为抵抗感染微生物的防御素的其他组分，包括类似于昆虫毒液过敏原的分子。来自该转录组的组分的连接表明A. cajennense蜱在摄食过程中，但强调在凝血系统。(c)2008年由Elsevier Ltd.出版
The neotropical tick Amblyomma cajennense is a significant pest to domestic animals, the most frequently human-biting tick in South America and the main vector of Brazilian spotted fever (caused by Rickettsia rickettsii), a deadly human disease. The purpose of this study is to characterize the adult A. cajennense salivary gland transcriptome by expressed sequence tags (ESTs). We report the analysis of 1754 clones obtained from a cDNA library, which reveal mainly transcripts related to proteins involved in the hemostatic processes, especially proteases and their inhibitors. Remarkably, five types of possible serine protease inhibitors were found, including a molecule with a distinguished structure that contains repeats of the active motif of hirudin inhibitors. Besides, other components that may be active over the host immune system or acting as defensins against infecting microorganisms were also described, including a molecule similar to insect venom allergens. The conjunction of components from this transcriptome suggests a diverse strategy of A. cajennense tick during feeding, but emphasized in the coagulation system. (c) 2008 Published by Elsevier Ltd.