tRNA-independent Pretransfer Editing by Class I Leucyl-tRNA Synthetase
tRNA-independent Pretransfer Editing by Class I Leucyl-tRNA Synthetase
复制标题
I 类亮氨酰-tRNA 合成酶进行不依赖 tRNA 的转移前编辑*
DOI：
10.1074/jbc.m806717200
复制
发表时间：
2009-02-06
影响因子：
4.8
通讯作者：
Wang, En-Duo
中科院分区：
文献类型：
--
作者：
Zhu, Bin;Yao, Peng;Wang, En-Duo
Aminoacyl-tRNA synthetases catalyze the formation of aminoacyl-tRNA in a two-step reaction starting with amino acid activation followed by aminoacyl group transfer to tRNA. To clear mistakes that occasionally occur, some of these enzymes carry out editing activities, acting on the misactivated amino acid (pretransfer editing) or after the transfer on the tRNA (post-transfer editing). The post-transfer editing pathway of leucyl-tRNA synthetase has been extensively studied by structural and biochemical approaches. Here, we report the finding of a tRNA-independent pretransfer editing pathway in leucyl-tRNA synthetases from Aquifex aeolicus. Using a CP1-mutant defective in its post-transfer editing function, we showed that this new editing pathway is distinct from the post-transfer editing site and may occur at the synthetic catalytic site, as recently proposed for other aminoacyl-tRNA synthetases.
