Comparison of the growth performance and long-chain polyunsaturated fatty acids (LC-PUFA) biosynthetic ability of red tilapia (Oreochromis mossambicus♀×O. niloticus♂) fed fish oil or vegetable oil diet at different salinities
Comparison of the growth performance and long-chain polyunsaturated fatty acids (LC-PUFA) biosynthetic ability of red tilapia (Oreochromis mossambicus♀×O. niloticus♂) fed fish oil or vegetable oil diet at different salinities
复制标题
不同盐度饲喂鱼油或植物油饲料的红罗非鱼（Oreochromis mossambicus—O. niloticus—）的生长性能和长链多不饱和脂肪酸（LC-PUFA）生物合成能力比较
DOI：
10.1016/j.aquaculture.2021.736899
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发表时间：
2021
期刊：
影响因子：
4.5
通讯作者：
Yuanyou Li
中科院分区：
文献类型：
--
作者：
Jun Yu;Xiaobo Wen;Cuihong You;Shuqi Wang;Cuiying Chen;Douglas R. Tocher;Yuanyou Li
比较了罗非鱼生长和长链多不饱和脂肪酸（LC-PUFA）生物合成能力的差异。niloticus niloticus）在不同盐度下的生长情况。用等氮（32%）和等脂（9%）两种饵料，在0、15和30 ppt盐度条件下对初始体重约10.5g的红罗非鱼幼鱼进行了为期8周的饲养试验。添加植物油的饲料被命名为VO，特别是菜籽油，不含LC-PUFA但富含C18 PUFA前体，另一种添加鱼油的饲料被命名为富含LC-PUFA的FO。试验结束时，无论投喂哪种饲料，30 ppt组的增重和特定生长率均显著高于15 ppt组和0 ppt组（p<0.05），而饲料转化率显著低于15 ppt组和0 ppt组（p<0.05）。此外，一些LC-PUFA显着影响水体盐度。在投喂VO饲料的鱼中，与投喂0 ppt饲料的鱼相比，投喂30 ppt和15 ppt饲料的鱼肝脏中二十碳五烯酸（EPA; 20：5 n-3）、二十二碳六烯酸（DHA，22：6 n-3）和总n-3 PUFA的比例显著较高（p<0.05），肌肉中DHA和花生四烯酸（ARA; 20：4 n-6）的比例显著较高（p<0.05）。此外，与LC-PUFA生物合成相关的基因（包括脂肪酰去饱和酶2（fads 2）和延伸酶5（elovl 5））的肝脏mRNA表达水平在30 ppt下饲养的鱼中显著高于在0 ppt下饲养的鱼（p<0.05）。这些结果表明，红罗非鱼具有从C18 PUFA前体生物合成LC-PUFA的能力，并且这种能力在30 ppt盐度下比在0 ppt盐度下更高，这可能与提高生长性能有关。
The present study aim to compare the growth and biosynthetic ability of long-chain polyunsaturated fatty acids (LC-PUFA) of red tilapia (Oreochromis mossambicus♀×O. niloticus♂) at different salinities. An 8-week feeding trial was conducted on the juvenile red tilapia (initial weight about 10.5 g) reared at 0, 15 and 30 parts per thousand (ppt) salinity fed with two isonitrogenous (32%) and isolipidic (9%) diets. The diet with vegetable oil was named VO, specifically rapeseed oil, without LC-PUFA but rich in C18PUFA precursors, the other diet with fish oil was named FO rich in LC-PUFA. At the end of feeding trial, irrespective of diet, fish reared at 30 ppt showed significantly higher weight gain and specific growth rate and lower feed conversion ratio than those reared at 15 ppt or 0 ppt (p<0.05). In addition, some LC-PUFA were significantly affected by water salinity. In fish fed the VO diet, proportions of eicosapentaenoic acid (EPA; 20:5n-3), docosahexaenoic acid (DHA, 22:6n-3) and total n-3 PUFA were significantly higher in liver (p<0.05), and DHA and arachidonic acid (ARA; 20:4n-6) significantly higher in muscle of fish reared at 30 ppt and 15 ppt than those reared at 0 ppt (p<0.05). Furthermore, liver mRNA expression levels of genes related to LC-PUFA biosynthesis including fatty acyl desaturase 2 (fads2) and elongase 5 (elovl5) were significantly higher in fish reared at 30 ppt than those reared at 0 ppt (p<0.05). These results indicated that red tilapia has the capability to biosynthesize LC-PUFA from C18PUFA precursors, and such ability was higher at 30 ppt salinity than in 0 ppt salinity, and this may be associated with improved growth performance.