Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis.
Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis.
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实验性鼠曲霉菌病肺部真菌负荷测定的三种方法的比较。
DOI：
10.1111/j.1469-0691.2005.01349.x
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Filler,SG
中科院分区：
文献类型：
--
作者：
Sheppard,DC;Marr,KA;Fredricks,DN;Chiang,LY;Doedt,T;Filler,SG
比较了定量培养、定量PCR和半乳甘露聚糖酶免疫分析法（EIA）测定侵袭性曲霉病小鼠模型肺真菌负荷的能力。与其他两种方法相比，含有菌丝的标本的定量培养不足以代表确定感染中的绝对真菌负荷。两种非培养方法的相关性最好。与定量PCR相比，半乳甘露聚糖EIA观察到更高的变异性。总的来说，这些数据表明，定量PCR是测定实验性曲霉病肺部真菌负荷的首选方法。
Quantitative culture, quantitative PCR and the galactomannan enzyme immunoassay (EIA) were compared for their ability to determine the pulmonary fungal burden in a murine model of invasive aspergillosis. Quantitative culture of specimens containing hyphae under‐represented the absolute fungal burden in established infection when compared with the two other methods. The best correlation was observed between the two non‐culture methods. Higher variability was observed with the galactomannan EIA when compared with quantitative PCR. Collectively, these data suggest that quantitative PCR is the preferred method for determination of the pulmonary fungal burden in experimental aspergillosis.