Phenotypic characterization of thymic prelymphoma cells of B10 mice treated with split-dose irradiation.
Phenotypic characterization of thymic prelymphoma cells of B10 mice treated with split-dose irradiation.
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分剂量照射处理的 B10 小鼠胸腺前淋巴瘤细胞的表型特征。
作者：
Masahiro Muto;Eiko Kubo;H. Kamisaku;T. Sado
对 B10 Thy-1 同系小鼠进行胸腺内注射测定，结果表明胸腺前淋巴瘤细胞首先在分次剂量照射后 4 至 8 天在胸腺内发育，并且在照射后 21 和 31 天检查时在超过 63% 的测试供体胸腺中检测到。此外，一些小鼠（25%）在分次剂量照射后 2 个月时，胸腺中已经出现胸腺淋巴瘤。为了表征这些胸腺淋巴瘤前细胞，用抗 CD4 和抗 CD8 mAb 对照射后 1 个月的 B10 Thy-1.1 小鼠的胸腺细胞进行染色，并将其分为四个亚群。将这些分级的细胞注射到受体胸腺中，以检查哪个亚群含有胸腺淋巴瘤前细胞。结果表明，胸腺淋巴瘤前细胞主要存在于CD4-CD8-和CD4-CD8+胸腺细胞亚群中，也存在CD4+CD8+亚群。源自CD4-CD8-前淋巴瘤细胞的T细胞淋巴瘤主要具有CD4-CD8-或CD4-CD8+表型。由CD4-CD8+淋巴瘤前细胞发展而来的T细胞淋巴瘤主要表达CD4-CD8+或CD4+CD8+表型。起源于CD4+CD8+淋巴瘤前细胞的T细胞淋巴瘤主要是CD4+CD8+，但也存在一些CD4-CD8+或CD4+CD8-细胞。这些胸腺前淋巴瘤细胞的表型进一步特征在于其标记物的表达，该标记物由抗 J11d 标记物和 TL-2（胸腺白血病）Ag 的 mAb 定义，该标记物在 B10.Thy-1.2 或 B10.Thy-1.1 株的正常胸腺细胞上不表达，但出现在致淋巴瘤性辐射小鼠的胸腺细胞上。结果表明，J11d+、TL-2+细胞中存在淋巴瘤前期细胞。
Using an intrathymic injection assay on B10 Thy-1 congenic mice, it was demonstrated that thymic prelymphoma cells first developed within the thymuses from 4 to 8 days after split-dose irradiation and were detected in more than 63% of the test donor thymuses when examined at 21 and 31 days after irradiation. Moreover, some mice (25%) at 2 mo after split-dose irradiation had already developed thymic lymphomas in their thymuses. To characterize these thymic prelymphoma cells, the thymocytes from B10 Thy-1.1 mice 1 mo after irradiation were stained with anti-CD4 and anti-CD8 mAb and were sorted into four subpopulations. These fractionated cells were injected into the recipient thymuses to examine which subpopulation contained thymic prelymphoma cells. The results indicated that thymic prelymphoma cells existed mainly in CD4- CD8- and CD4- CD8+ thymocyte subpopulations and also in CD4+ CD8+ subpopulation. T cell lymphomas derived from CD4- CD8- prelymphoma cells had mainly CD4- CD8- or CD4- CD8+ phenotypes. T cell lymphomas developed from CD4- CD8+ prelymphoma cells mainly expressed CD4- CD8+ or CD4+ CD8+ phenotype. T cell lymphomas originating from CD4+ CD8+ prelymphoma cells were mainly CD4+ CD8+ but some CD4- CD8+ or CD4+ CD8- cells were also present. These thymic prelymphoma cells were further characterized phenotypically in relation to their expression of the marker defined by the mAb against J11d marker and TL-2 (thymus-leukemia) Ag, which is not expressed on normal thymocytes of B10.Thy-1.2 or B10.Thy-1.1 strain, but appears on the thymocytes of lymphomagenic irradiated mice. The results indicated that the prelymphoma cells existed in J11d+, TL-2+ cells.