Resorbable bioscaffold for esophageal repair in a dog model
Resorbable bioscaffold for esophageal repair in a dog model
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DOI：
10.1053/jpsu.2000.7834
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发表时间：
2000-07-01
影响因子：
2.4
通讯作者：
Simmons-Byrd, A
中科院分区：
文献类型：
--
作者：
Badylak, S;Meurling, S;Simmons-Byrd, A
目的：利用猪源性小肠粘膜下层（SIS）或膀胱粘膜下层（UBS）的异种细胞外基质（ECM）作为组织支架用于犬食管修复模型。方法：对健康成年母狗进行手术切除，形成长度约为5cm、约占食道周长40% ~ 50%的斑块缺损或长度为5cm的完整的周向节段缺损。用SIS或UBS衍生的ECM支架修复缺损。这些动物的存活时间从4天到15个月不等。结果：用于补片缺损修复的异种支架在30 ~ 60天内被完全吸收，并被骨骼肌替代，骨骼肌定向适当，与邻近正常食管骨骼肌相邻，胶原结缔组织组织有序，鳞状上皮完整完整。没有临床食道功能障碍的迹象，在任何动物的补丁缺陷修复。异种支架配置成管状修复节段性缺损，均在术后45天出现狭窄。结论：这些ecm有望作为食管修复的治疗选择，但对于全管移植来说，狭窄仍然是一个问题。版权所有(C) 2000由W.B. Saunders公司。
Purpose: Porcine-derived, xenogeneic extracellular matrix (ECM) derived from either the small intestinal submucosa (SIS) or urinary bladder submucosa (UBS) was used as a tissue scaffold for esophageal repair in a dog model.Methods: Patch defects measuring approximately 5 cm in length and encompassing 40% to 50% of the circumference of the esophagus or complete circumferential segmental defects measuring 5 cm in length were created by surgical resection in healthy adult female dogs. The defects were repaired with ECM scaffolds derived from either SIS or UBS. The animals were kept alive for periods ranging from 4 days to 15 months.Results: The xenogeneic scaffolds used for repair of the patch defects were resorbed completely within 30 to 60 days and showed replacement by skeletal muscle, which was oriented appropriately and contiguous with adjacent normal esophageal skeletal muscle, organized collagenous connective tissue, and a complete and intact squamous epithelium. No signs of clinical esophageal dysfunction were seen in any of the animals with the patch defect repair. The xenogeneic scaffolds configured into tubes for repair of the segmental defects all showed stricture within 45 days of surgery.Conclusion: These ECMs show promise as a treatment option for esophageal repair, but stricture remains problematic for complete tube grafts. Copyright (C) 2000 by W.B. Saunders Company.