Contribution of time of day and the circadian clock to the heat stress responsive transcriptome in Arabidopsis
Contribution of time of day and the circadian clock to the heat stress responsive transcriptome in Arabidopsis
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DOI：
10.1038/s41598-019-41234-w
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发表时间：
2019-03-18
影响因子：
4.6
通讯作者：
Nagel, Dawn H.
中科院分区：
文献类型：
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作者：
Blair, Emily J.;Bonnot, Titouan;Nagel, Dawn H.
在拟南芥中，一个大的热响应基因的子集表现出昼夜或昼夜振荡。然而，时间和/或生物钟的维度在多大程度上有助于热应激反应仍然是未知的。为了确定一天中的时间和/或生物钟对不同热应激反应的直接贡献，我们在清晨（ZT 1）和下午（ZT 6）暴露于热（37摄氏度）和中度冷（10摄氏度）后探测了生物钟基因CCA 1，LHY，PRR 7和PRR 9的野生型和突变体。数以千计的基因在响应温度、一天中的时间和/或时钟突变时差异表达。与上午相比，下午多了大约30%的基因差异表达，热应激显著干扰了转录组。在特异性响应于热应激的DEG（类似于3000）中，类似于70%显示转录响应的一天中的时间（ZT 1或ZT 6）发生。对于在ZT 1和ZT 6之间共享的DEG（类似于1400），我们观察到转录响应的幅度的变化。此外，约2%的DEG对温度胁迫表现出不同的反应。这项研究的结果强调了一天中的时间在热应激反应转录组中的重要作用，并且通过CCA 1和LHY的时钟似乎比PRR 7和PRR 9在调节白天的热应激反应中具有更深刻的作用。我们的研究结果强调了在拟南芥非生物胁迫响应研究中考虑时间维度的重要性。
In Arabidopsis, a large subset of heat responsive genes exhibits diurnal or circadian oscillations. However, to what extent the dimension of time and/or the circadian clock contribute to heat stress responses remains largely unknown. To determine the direct contribution of time of day and/or the clock to differential heat stress responses, we probed wild-type and mutants of the circadian clock genes CCA1, LHY, PRR7, and PRR9 following exposure to heat (37 degrees C) and moderate cold (10 degrees C) in the early morning (ZT1) and afternoon (ZT6). Thousands of genes were differentially expressed in response to temperature, time of day, and/or the clock mutation. Approximately 30% more genes were differentially expressed in the afternoon compared to the morning, and heat stress significantly perturbed the transcriptome. Of the DEGs (similar to 3000) specifically responsive to heat stress, similar to 70% showed time of day (ZT1 or ZT6) occurrence of the transcriptional response. For the DEGs (similar to 1400) that are shared between ZT1 and ZT6, we observed changes to the magnitude of the transcriptional response. In addition, similar to 2% of all DEGs showed differential responses to temperature stress in the clock mutants. The findings in this study highlight a significant role for time of day in the heat stress responsive transcriptome, and the clock through CCA1 and LHY, appears to have a more profound role than PRR7 and PRR9 in modulating heat stress responses during the day. Our results emphasize the importance of considering the dimension of time in studies on abiotic stress responses in Arabidopsis.