Recent progress in the development of derivatization reagents having a benzofurazan structure.
Recent progress in the development of derivatization reagents having a benzofurazan structure.
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DOI：
10.1002/bmc.945
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发表时间：
2008-04
期刊：
影响因子：
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通讯作者：
T. Santa;T. Fukushima;Tomoko Ichibangase;K. Imai
中科院分区：
文献类型：
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作者：
T. Santa;T. Fukushima;Tomoko Ichibangase;K. Imai
在高效液相色谱（HPLC）中，为了提高目标化合物的检测灵敏度和分离效率，常采用化学衍生化方法。本文综述了近年来苯并呋咱衍生化试剂的研究进展，包括荧光试剂、水溶性试剂、多肽和蛋白质分析试剂以及质谱检测试剂。并简要介绍了这些试剂在生物样品中的应用。
Chemical derivatization is often used to enhance the detectability of the target compounds and to improve the separation efficiency in high-performance liquid chromatography (HPLC). In this review, we describe the recent progress in the development of derivatization reagents having a benzofurazan structure, namely, the fluorogenic reagents, water-soluble reagents, reagents for the analysis of peptides and proteins, and reagents for mass spectrometric detection. The application of these reagents to bio-samples is also briefly described.