Structural analysis of unstable norbixin isomers guided by pure shift nuclear magnetic resonance
Structural analysis of unstable norbixin isomers guided by pure shift nuclear magnetic resonance
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DOI：
10.1002/mrc.5252
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发表时间：
2022-02-07
影响因子：
2
通讯作者：
Giraud, Nicolas
中科院分区：
文献类型：
--
作者：
Bertho, Gildas;Oumezziane, Imed Eddine;Giraud, Nicolas
我们报告的复杂样品的分析过程中获得的微波辐射/加热的norbixin，这已被确定为一个潜在的治疗目标，年龄相关性黄斑变性（AMD）。在这方面，确定在其降解过程中获得的不同异构体至关重要。然而，这种表征是具有挑战性的，因为，一方面，这些异构体中的一些是不稳定的，另一方面，这些异构体混合物的H-1光谱分辨率差。我们可以成功地应用1D纯位移实验，以获得超高分辨率的H-1核磁共振（NMR）谱的降胭脂树红异构体样品，并利用其信息内容来分析互补的2D NMR数据和准确地描述其异构体组成。
We report the analysis of complex samples obtained during the microwave irradiation/heating of norbixin, which has been identified as a potential therapeutic target for age-related macular degeneration (AMD). In this context, identifying the different isomers that are obtained during its degradation is of primary importance. However, this characterization is challenging because, on the one hand, some of these isomers are unstable, and on the other hand, the H-1 spectra of these isomeric mixtures are poorly resolved. We could successfully apply 1D pure shift experiments to obtain ultrahigh-resolution H-1 nuclear magnetic resonance (NMR) spectra of the norbixin isomer samples and exploit their information content to analyze complementary 2D NMR data and describe accurately their isomeric composition.