Structure of the mouse lysyl oxidase gene.
Structure of the mouse lysyl oxidase gene.
复制标题
小鼠赖氨酰氧化酶基因的结构。
DOI：
10.1006/geno.1993.1202
复制
发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Friedman,RM
中科院分区：
文献类型：
--
作者：
Contente,S;Csiszar,K;Kenyon,K;Friedman,RM
赖氨酰氧化酶是一种参与胶原蛋白和弹性蛋白成熟的胞外酶，似乎也是一种表型抑制基因产物p21转化的抑制因子。小鼠赖氨酰氧化酶基因的基因组克隆已被分离、分析和测序。赖氨酰氧化酶似乎是一个单拷贝基因，由7个外显子和6个内含子组成，跨越小鼠基因组约14 kb。该基因编码两种信息，大小约为4.8和3.8 kb，在基因3′端的非翻译序列长度不同。所有的3′非翻译序列和多聚腺苷酸化信号都包含在外显子VII中;没有证据表明存在选择性剪接。引物延伸和核糖核酸酶保护实验揭示了在具有启动子特征序列基序的区域中的两个转录起始位点，该区域位于cDNA中发现的5′非翻译序列的上游和邻近。
Lysyl oxidase, an extracellular enzyme involved in the maturation of collagen and elastin, also appears to function as a phenotypic suppressor of transformation by therasgene product, p21. Genomic clones of the mouse lysyl oxidase gene have been isolated, analyzed, and sequenced. Lysyl oxidase appears to be a single-copy gene, organized into seven exons and six introns, and spans approximately 14 kb of the mouse genome. The gene encodes two messages, sized at about 4.8 and 3.8 kb, that differ in the length of the untranslated sequence at the 3′ end of the gene. All of the 3′ untranslated sequence and the polyadenylation signals are contained in exon VII; there is no evidence of alternate splicing. Primer extension and ribonuclease protection experiments revealed two sites of transcription initiation in a region with sequence motifs characteristic of a promoter, which was upstream and adjacent to the 5′ untranslated sequence found in the cDNA.