Self-assembled signaling aptamer DNA arrays for protein detection
Self-assembled signaling aptamer DNA arrays for protein detection
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DOI：
10.1002/anie.200600438
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发表时间：
2006-01-01
影响因子：
16.6
通讯作者：
Yan, Hao
中科院分区：
文献类型：
--
作者：
Lin, Chenxiang;Katilius, Evaldas;Yan, Hao
在这里，我们报道了一种DNA磁片，它直接将信号适配子自组装成高密度纳米阵列，用于高度灵敏的蛋白质分子检测。显示在自组装DNA阵列上的信号适配子的高局部密度使我们能够使用共聚焦荧光显微镜成像来检测与适配子结合的亚纳摩尔浓度的蛋白质。这种水溶性适体微阵列为开发可编程传感器阵列提供了一种新的策略。
Here we report a DNA tile directed self-assembly of signaling aptamers into high-density nanoarrays for highly sensitive detection of protein molecules. The high local density of the signaling aptamers displayed on the self-assembled DNA arrays allowed us to detect sub-nanomolar concentrations of protein bound to the aptamers using confocal fluorescence microscope imaging. This water-soluble aptamer microarray provides a novel strategy for developing programmable sensor arrays.