QTL identification for salt tolerance related traits at the seedling stage inindicarice using a multi-parent advanced generation intercross (MAGIC) population
QTL identification for salt tolerance related traits at the seedling stage inindicarice using a multi-parent advanced generation intercross (MAGIC) population
复制标题
利用多亲高代杂交 (MAGIC) 群体对籼稻苗期耐盐相关性状进行 QTL 鉴定
DOI：
10.1007/s10725-020-00644-x
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发表时间：
2020
影响因子：
4.2
通讯作者：
Ye Guoyou
中科院分区：
文献类型：
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作者：
Zhang Ya;Ponce Kimberly S.;Meng Lijun;Chakraborty Panchali;Zhao Qingyuan;Guo Longbiao;Gao Zhenyu;Leng Yujia;Ye Guoyou
盐胁迫是影响水稻生产的重要非生物胁迫之一。本研究以4个优良品种杂交获得的多亲本高世代互交群体DC 1为材料，对耐盐性QTL进行了定位。用55 K水稻SNP芯片对整个群体和4个亲本进行基因分型。在186个显著的标记-性状关联中，共检测到7个QTL，分布在第1、2、5和9染色体上，占总表型变异的7.42-9.38%。在这些QTL中，检测到1个位于第2染色体上的新QTL（qRRL 2）和1个位于第1染色体上的多性状QTL（qSLST 1/qRDSW 1/qRB 1），分别控制盐胁迫下的地上部长度、根干重和根生物量。基因表达分析表明，位于多性状QTL内的转录因子基因（LOC_Os01g66280）是潜在的耐盐候选基因。有趣的是，我们在这个候选基因的编码区发现了一个显著的非同义SNP。这些结果将有助于对该候选基因的精细定位和QTL定位，为水稻耐盐性的遗传改良奠定基础。
Salinity is one of the most important abiotic stresses, which seriously affects rice production. In this study, a multiparent advanced generation intercross population, DC1, derived from intercrossing four eliteindicavarieties, was used to identify QTLs conferring salt tolerance. The whole population and four parents were genotyped with a 55K rice SNP array. A total of 7 QTLs delineated from 186 significant marker-trait associations were detected on chromosomes 1, 2, 5 and 9, which accounted for 7.42–9.38% of the total phenotypic variations. Among these QTLs, one novel QTL (qRRL2) on chromosome 2 for relative root length and one multi-trait QTL (qSLST1/qRDSW1/qRB1) on chromosome 1 affecting shoot length, root dry weight and root biomass under salt treatment were detected. Gene expression analysis revealed that a transcription factor gene (LOC_Os01g66280) within the multi-trait QTL is the potential candidate gene for salt tolerance. Interestingly, we identified one significant nonsynonymous SNP in the coding region of this candidate gene. These results will facilitate fine mapping of the candidate gene and QTL pyramiding to genetically improve salt tolerance in rice.