Evolution of plant δ(1)-pyrroline-5-carboxylate reductases from phylogenetic and structural perspectives.
Evolution of plant δ(1)-pyrroline-5-carboxylate reductases from phylogenetic and structural perspectives.
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DOI：
10.3389/fpls.2015.00567
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发表时间：
2015
影响因子：
5.6
通讯作者：
Nocek B
中科院分区：
文献类型：
--
作者：
Forlani G;Makarova KS;Ruszkowski M;Bertazzini M;Nocek B
脯氨酸在细胞生长和逆境反应中起着至关重要的作用，其积累对于植物对不利环境条件的耐受是必不可少的。在植物中生物合成脯氨酸有两条途径。主要路线使用谷氨酸作为前体，而在另一条路线中，脯氨酸来自鸟氨酸。这两个途径的最后一步，δ1-吡咯烷-5-羧酸(P5C)转化为L-脯氨酸，是由P5C还原酶(P5CR)催化的，辅因子为NADH或NADPH。由于P5CR是重要的管家酶，它们在生命的所有领域都是保守的，在整个进化过程中似乎相对不受影响。然而，对这些酶的全球分析揭示了在对辅因子(NADPH与NADH)的偏好、金属依赖性的变化以及寡聚体状态的差异方面存在显著的功能差异。在我们的研究中，我们通过对来自所有生命王国的P5CR代表的系统发育和结构分析来研究进化模式，重点是植物物种。我们还试图将该家族的功能和结构特征的成员之间的局部序列/结构变异联系起来。
Proline plays a crucial role in cell growth and stress responses, and its accumulation is essential for the tolerance of adverse environmental conditions in plants. Two routes are used to biosynthesize proline in plants. The main route uses glutamate as a precursor, while in the other route proline is derived from ornithine. The terminal step of both pathways, the conversion of δ1-pyrroline-5-carboxylate (P5C) to L-proline, is catalyzed by P5C reductase (P5CR) using NADH or NADPH as a cofactor. Since P5CRs are important housekeeping enzymes, they are conserved across all domains of life and appear to be relatively unaffected throughout evolution. However, global analysis of these enzymes unveiled significant functional diversity in the preference for cofactors (NADPH vs. NADH), variation in metal dependence and the differences in the oligomeric state. In our study we investigated evolutionary patterns through phylogenetic and structural analysis of P5CR representatives from all kingdoms of life, with emphasis on the plant species. We also attempted to correlate local sequence/structure variation among the functionally and structurally characterized members of the family.