Buffer-induced swelling and vesicle budding in binary lipid mixtures of dioleoylphosphatidylcholine:dioleoylphosphatidylethanolamine and dioleoylphosphatidylcholine:lysophosphatidylcholine using small-angle X-ray scattering and ³¹P static NMR.
Buffer-induced swelling and vesicle budding in binary lipid mixtures of dioleoylphosphatidylcholine:dioleoylphosphatidylethanolamine and dioleoylphosphatidylcholine:lysophosphatidylcholine using small-angle X-ray scattering and ³¹P static NMR.
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使用小角 X 射线散射和 3P 静态 NMR，在二油酰磷脂酰胆碱：二油酰磷脂酰乙醇胺和二油酰磷脂酰胆碱：溶血磷脂酰胆碱的二元脂质混合物中缓冲液诱导的肿胀和囊泡出芽。
DOI：
10.1021/la5047996
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Barriga HM
中科院分区：
文献类型：
--
作者：
Barriga HM
存在大量关于脂质相行为的数据；然而，它主要是在非生物温度范围内的非缓冲系统中。我们提供了二油酰磷脂酰胆碱 (DOPC)、二油酰磷脂酰乙醇胺 (DOPE) 和溶血磷脂酰胆碱 (LysoPC) 脂质混合物的生物物理数据，检查它们在过量水和缓冲系统中在 4-34 °C 温度范围内的行为。这些混合物通常用于研究自发曲率对完整膜蛋白的影响。使用小角 X 射线散射 (SAXS) 和 31 P NMR，我们观察到层状和囊泡相，缓冲液导致层间距增加。 DOPC 双层中 DOPE 量的增加会减小中间相的层间距，而 LysoPC 量的增加则观察到相反的趋势。使用 31P 静态 NMR 分析 DOPC:LysoPC 样品以研究存在的囊泡大小，并在 LysoPC 浓度高于 30 mol% 时观察到囊泡出芽的证据。使用适应的程序来拟合核磁共振线形状，该程序考虑了磁场内脂质的变形。由于磁化率，囊泡的变形随 LysoPC 含量的变化而变化，并且在水和缓冲液样品中都发现了不连续性。一般来说，畸变随着LysoPC含量的增加而增加；然而，当 DOPC:LysoPC 的比例为 60:40 时，样品显示出与纯 DOPC 相似的囊泡变形程度。这意味着此时膜的柔韧性增加。通常，假设随着 LysoPC 浓度的增加，膜张力会降低，这意味着基于自发曲率假设的膜张力估计可能不准确。
A large variety of data exists on lipid phase behavior; however, it is mostly in nonbuffered systems over nonbiological temperature ranges. We present biophysical data on lipid mixtures of dioleoylphosphatidylcholine (DOPC), dioleoylphosphatidylethanolamine (DOPE), and lysophosphatidylcholine (LysoPC) examining their behaviors in excess water and buffer systems over the temperature range 4–34 °C. These mixtures are commonly used to investigate the effects of spontaneous curvature on integral membrane proteins. Using small-angle X-ray scattering (SAXS) and31P NMR, we observed lamellar and vesicle phases, with the buffer causing an increase in the layer spacing. Increasing amounts of DOPE in a DOPC bilayer decreased the layer spacing of the mesophase, while the opposite trend was observed for increasing amounts of LysoPC.31P static NMR was used to analyze the DOPC:LysoPC samples to investigate the vesicle sizes present, with evidence of vesicle budding observed at LysoPC concentrations above 30 mol %. NMR line shapes were fitted using an adapted program accounting for the distortion of the lipids within the magnetic field. The distortion of the vesicle, because of magnetic susceptibility, varied with LysoPC content, and a discontinuity was found in both the water and buffer samples. Generally, the distortion increased with LysoPC content; however, at a ratio of DOPC:LysoPC 60:40, the sample showed a level of distortion of the vesicle similar to that of pure DOPC. This implies an increased flexibility in the membrane at this point. Commonly, the assumption is that for increasing LysoPC concentration there is a reduction in membrane tension, implying that estimations of membrane tension based on spontaneous curvature assumptions may not be accurate.
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影响因子：
6
作者：
Nucci NV;Vanderkooi JM
通讯作者：
Vanderkooi JM
溶血磷脂酰胆碱与磷脂酰胆碱双层的相互作用。
DOI：
10.1016/0005-2736(80)90185-6
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Morris,DA;McNeil,R;Castellino,FJ;Thomas,JK
通讯作者：
Thomas,JK
第 3 章 - 脂水系统的多态性
DOI：
--
发表时间：
1995
期刊：
影响因子：
--
作者：
J. Seddon;R. Templer
通讯作者：
R. Templer
影响因子：
3.4
作者：
Powl, Andrew M.;East, J. Malcolm;Lee, Anthony G.
通讯作者：
Lee, Anthony G.
31P核磁共振和膜中磷脂的头基结构。
DOI：
--
发表时间：
1978
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
Joachim Seelig
通讯作者：
Joachim Seelig