Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed
Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed
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DOI：
10.1111/j.1439-0523.2007.01435.x
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发表时间：
2008-02-01
期刊：
影响因子：
2
通讯作者：
He, Q. B.
中科院分区：
文献类型：
--
作者：
Hong, D. F.;Liu, J.;He, Q. B.
Rs1046AB是油菜显性核不育系，其不育性一直被认为是由雄性不育基因(Ms)及其非等位恢复基因(Rf)互作控制的。该系统不仅为轮回选择提供了辅助工具，而且为杂交制种提供了一个很有前途的系统。在前人研究的基础上，将两个与ms基因连锁的扩增片段长度多态标记分别转化为显性和共显性序列特征扩增区(SCAR)标记。3个与RF基因遗传距离相同的显性SCAR标记之间的线性顺序关系也是通过检查这些标记的同源物是否存在于携带RF的不同品系中来确定的。与Rf基因连锁最近的标记在所有携带隐性等位基因Rf的品系中都产生了一个较大的片段，这表明该标记是一个共显性标记，并通过这些片段的核苷酸序列比较进一步证实了这一点。MS和Rf特异的SCAR标记在分子标记辅助DGMS三系育种中具有重要的应用价值。
Rs1046AB is a dominant genic male sterility (DGMS) line in rapeseed, in which the sterility has always been thought to be conditioned by the interaction of a male sterility gene (Ms) and its non-allelic restorer gene (Rf). This system provides not only a tool for assisting in recurrent selection but also a promising system for hybrid production. Based on previous studies, two amplified fragment length polymorphism markers linked with the Ms gene were converted into a dominant and a co-dominant sequence characterized amplified region (SCAR) marker, respectively. The putative linear order relationship of three dominant SCAR markers with the same genetic distance from the Rf gene, was also determined by an examination of whether the homologues of these markers are present or not in different lines carrying Rf. A bigger fragment generated by the closest marker linked to the Rf gene was observed in all lines carrying the recessive allele rf, suggesting that this marker is a co-dominant marker, which was further confirmed by nucleotide sequence comparison of these fragments. SCAR markers specific for Ms and Rf will be especially valuable in marker-assisted DGMS three-line breeding.