Application of a real-time biosensor to detect bacteria in platelet concentrates.
Application of a real-time biosensor to detect bacteria in platelet concentrates.
复制标题
应用实时生物传感器检测浓缩血小板中的细菌。
DOI：
10.1016/s0006-291x(02)02828-0
复制
发表时间：
2003
影响因子：
3.1
通讯作者：
Cote,MindyA
中科院分区：
文献类型：
--
作者：
Rotman,Boris;Cote,MindyA
一种基于孢子的实时检测低水平细菌的生物传感器最近被开发出来。该系统（称为LEXSAS，无标签指数信号放大系统）利用孢子在感应邻近细菌细胞时产生荧光的能力。我们研究了LEXSAS作为输血前鉴定细菌污染血小板浓缩物的可能方法，因为该系统提供快速分析、高灵敏度和低成本。如果成功，这种方法可以降低输血相关菌血症和败血症的发病率和死亡率。在这项研究中，我们使用LEXSAS检测含有蜡样芽孢杆菌、阴沟肠杆菌、大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌或化脓性链球菌的血小板浓缩物中的细菌。根据细菌对洗涤剂和渗透休克的抗性，使用2分钟的程序从血小板中分离细菌。结果表明，LEXSAS可用于设计一种实用的生物传感器，用于实时识别细菌污染的血小板。
A spore-based biosensor for detecting low levels of bacteria in real-time has been recently developed. The system (termed LEXSAS, label-free exponential signal-amplification system) exploits spore’s ability to produce fluorescence when sensing neighboring bacterial cells. We studied the LEXSAS as a possible approach for identifying bacterially contaminated platelet concentrates prior to transfusion because the system offers rapid analysis, high sensitivity, and low cost. If successful, this approach could reduce the risk of morbidity and mortality from transfusion-related bacteremia and sepsis. In this study, we used the LEXSAS for detecting bacteria in platelet concentrates spiked with Bacillus cereus, Enterobacter cloacae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, or Streptococcus pyogenes. Bacteria were separated from platelets using a 2-min procedure based on bacterial resistance to detergents and osmotic shock. The results indicate that the LEXSAS could be used to design a practical biosensor for identifying bacterially contaminated platelets in real-time.