Prenatal diagnosis of fetal cytomegalovirus infection in seropositive pregnant women
Prenatal diagnosis of fetal cytomegalovirus infection in seropositive pregnant women
复制标题
DOI：
10.1016/0301-2115(95)02541-3
复制
发表时间：
1996-11-01
期刊：
影响因子：
--
通讯作者：
Souliou, E
中科院分区：
文献类型：
--
作者：
Kyriazopoulou, V;Bondis, J;Souliou, E
对32例妊娠前对人巨细胞病毒（HCMV）免疫的孕妇进行了子宫颈穿刺和胎儿HCMV感染的联合采血检查。孕妇无反复感染史，超声检查均正常。采用酶免疫法和聚合酶链反应（PCR）检测孕妇血清和羊水中抗HCMV抗体和病毒DNA。所有孕妇血清病毒DNA和IgM抗HCMV抗体均为阴性。但在4例孕妇的羊水中检测到HCMV DNA。这4例病例被认为是胎儿HCMV感染。其中3例在出生后进行了随访。这三个新生儿出生时临床表现正常，但在他们的尿液标本和其中两个在脐带血血清中检测到HCMV DNA。虽然...新生儿出生时无症状，不能排除日后出现后遗症的可能性。结果表明，HCMV反复母体感染是一种隐匿性感染，不易被怀疑和诊断。他们还表明，胎儿HCMV感染在免疫前孕妇中并不罕见。
Thirty two pregnant women, immune to human cytomegalovirus (HCMV) before conception, were examined with a combination of amniocentesis, and blood sampling for fetal HCMV infection. The pregnant women had no history of recurrent infection and they all had normal ultrasonographic findings. Maternal sera and amniotic fluids were examined for anti-HCMV antibodies and viral DNA by enzyme immunoassay and polymerase chain reaction (PCR). All maternal sera were negative for viral DNA and IgM anti-HCMV antibodies. However, HCMV DNA was detected in the amniotic fluid of four pregnant women. These four cases were considered as fetal HCMV infections. Three of them were followed up after birth. The three neonates appeared clinically normal at birth, but HCMV DNA was detected in their urine specimens and in two of them in the cord blood sera. Although. the neonates were asymptomatic at birth, the possibility of developing sequelae later on, cannot be excluded. The results show that HCMV recurrent maternal infection is silent and difficult to be suspected and diagnosed. They also show that fetal HCMV infection is not rare among pre-immune pregnant women.