Ultrasensitive DNA detection using oligonucleotide-silver nanoparticle conjugates
Ultrasensitive DNA detection using oligonucleotide-silver nanoparticle conjugates
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DOI：
10.1021/ac702403w
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发表时间：
2008-04-15
影响因子：
7.4
通讯作者：
Graham, Duncan
中科院分区：
文献类型：
--
作者：
Thompson, David G.;Enright, Alexis;Graham, Duncan
寡核苷酸-金纳米颗粒（OGN）偶联物由于纳米颗粒赋予寡核苷酸的独特性质而成为检测靶DNA序列的有力工具。实际上，这些缀合物的所有研究和应用都使用了金纳米颗粒而排除了其他贵金属纳米颗粒。在这里，我们报告的合成的利奈肽-银纳米粒子（OSN）共轭物，并证明其使用的夹心测定格式。OSN缀合物与其金类似物具有几乎相同的性质，并且由于其极大的消光系数，可见和吸收分析都可以在低得多的浓度下进行。这是OSN缀合物成功用于靶DNA检测的第一份报告，并提供了一系列科学家感兴趣的改进的灵敏度。
Oligonucleotide-gold nanoparticle (OGN) conjugates are powerful tools for the detection of target DNA sequences due to the unique properties conferred upon the oligonucleotide by the nanoparticle. Practically all the research and applications of these conjugates have used gold nanoparticles to the exclusion of other noble metal nanoparticles. Here we report the synthesis of oligonucleotide-silver nanoparticle (OSN) conjugates and demonstrate their use in a sandwich assay format. The OSN conjugates have practically identical properties to their gold analogues and due to their vastly greater extinction coefficient both visual and absorption analyses can occur at much lower concentrations. This is the first report of OSN conjugates being successfully used for target DNA detection and offers improved sensitivity which is of interest to a range of scientists.