DETECTION OF LATENCY-RELATED VIRAL RNAS IN TRIGEMINAL GANGLIA OF RABBITS LATENTLY INFECTED WITH HERPES-SIMPLEX VIRUS TYPE-1
DETECTION OF LATENCY-RELATED VIRAL RNAS IN TRIGEMINAL GANGLIA OF RABBITS LATENTLY INFECTED WITH HERPES-SIMPLEX VIRUS TYPE-1
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DOI：
10.1128/jvi.61.12.3820-3826.1987
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发表时间：
1987-12-01
影响因子：
5.4
通讯作者：
WECHSLER, SL
中科院分区：
文献类型：
--
作者：
ROCK, DL;NESBURN, AB;WECHSLER, SL
采用原位杂交和北方（RNA）印迹分析相结合，我们调查了单纯疱疹病毒1型（HSV-1）的转录活性在一个眼部模型的HSV-1潜伏期。将放射性标记的克隆片段（实际上代表整个HSV-1基因组）分别与HSV-1感染潜伏期家兔三叉神经节切片中的RNA杂交（McKrae）。我们的研究结果表明，两个离散的潜伏期相关的RNA（LR-RNA）可能存在。LR-RNA主要定位于神经元细胞核。更丰富的LR-RNA检测到约3%的所有神经元检查，并指定为主要LR-RNA。另一种LR-RNA，命名为次要LR-RNA，在潜伏感染兔的约0.3%的神经元中检测到。LR-RNA的基因定位在立即早期基因ICP 0（也称为IE110）附近。主要LR-RNA的基因与ICP 0基因的左端（3“）部分重叠。用单链RNA探针进行原位杂交，结果表明该LR-RNA与ICP 0 mRNA具有互补的意义。北方印迹分析给出该LR-RNA的大约大小为1.8至2.2个内切酶。次要LR-RNA定位于或接近ICP 0基因的右端（5“）。LR-RNA的检测表明这些RNA或其产物可能在HSV-1潜伏期的起始和/或维持中起重要作用。
Using a combination of in situ hybridization and Northern (RNA) blot analysis, we investigated herpes simplex virus type 1 (HSV-1) transcriptional activity in an ocular rabbit model of HSV-1 latency. Radioactively labeled cloned fragments, representing virtually the entire HSV-1 genome, were individually hybridized to RNA in sections of trigeminal ganglia taken from rabbits during the latent phase of infection with HSV-1 (McKrae). Our results suggest that two discrete latency-related RNAs (LR-RNAs) may be present. The LR-RNAs were localized mainly in the nuclei of neurons. The more abundant LR-RNA was detected in approximately 3% of all neurons examined and was designated major LR-RNA. The other LR-RNA, designated minor LR-RNA, was detected in approximately 0.3% of neurons from latently infected rabbits. The genes for the LR-RNAs mapped in the vicinity of the immediate-early gene ICP0 (also designated IE110). The gene for the major LR-RNA partially overlapped the left (3'') end of the ICP0 gene. In situ hybridization with single-stranded RNA probes showed that this LR-RNA was of complementary sense to that of ICP0 mRNA. Northern blot analysis gave an approximate size for this LR-RNA of 1.8 to 2.2 kilobases. The minor LR-RNA mapped to or near the right (5'') end of the ICP0 gene. The detection of LR-RNAs suggests the possibility that these RNAs or their products may play significant roles in the initiation and/or maintenance of HSV-1 latency.