Nanoscale investigation of pathogenic microbial adhesion to a biomaterial
Nanoscale investigation of pathogenic microbial adhesion to a biomaterial
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DOI：
10.1128/aem.70.10.6012-6022.2004
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发表时间：
2004-10-01
影响因子：
4.4
通讯作者：
Camesano, TA
中科院分区：
文献类型：
--
作者：
Emerson, RJ;Camesano, TA
仅在美国，每年就有超过200万例手术病例发生医疗植入物的微生物感染。这些增加了患者发病率和死亡率，以及患者成本和恢复时间。有许多治疗方法，但没有一种能保证消除感染。在许多情况下，器械感染是由微生物粘附在植入物上，随后生长、发病和传播引起的。这项工作的目的是研究微生物粘附的初始事件，通过模拟的方法和接触之间的粘附细胞，固定在一个原子力显微镜（AFM）的悬臂梁，和生物材料或生物膜基板。本研究中使用的两种模式微生物，近平滑念珠菌（ATCC 90018）和铜绿假单胞菌（ATCC 10145），是根据其临床相关性以及在实验室环境中易于采集和处理而选择的。C.近平滑和未改性的硅橡胶和铜绿假单胞菌生物膜。利用C.通过使用固定在具有硅酮基底的AFM杠杆上的近平滑细胞，我们在力循环的接近部分中测量到4.3 +/-0.25nN的吸引力。在铜绿假单胞菌生物膜上，吸引力的大小降低至2.0 +/- 0.40 nN，并且在距离细胞表面约75 nm处的2.0-nN排斥力之前。这些数据表明C.近平滑可能粘附在硅橡胶和铜绿假单胞菌生物膜上，可能导致患者发病率和死亡率。细胞-生物材料和细胞-细胞相互作用的表征允许植入物材料的物理力学和物理化学性质与导致微生物定植和感染的纳米级相互作用之间的定量联系。
Microbial infections of medical implants occur in more than 2 million surgical cases each year in the United States alone. These increase patient morbidity and mortality, as well as patient cost and recovery time. Many treatments are available, but none are guaranteed to remove the infection. In many cases, the device infections are caused by the adhesion of microbes to the implant, ensuing growth, pathogenesis, and dissemination. The purpose of this work is to examine the initial events in microbial adhesion by simulating the approach and contact between a planktonic cell, immobilized on an atomic force microscope (AFM) cantilever, and a biomaterial or biofilm substrate. The two model microbes used in this study, Candida parapsilosis (ATCC 90018) and Pseudomonas aeruginosa (ATCC 10145), were chosen for both their clinical relevance and their ease of acquisition and handling in the laboratory setting. Attractive interactions exist between C. parapsilosis and both unmodified silicone rubber and P. aeruginosa biofilms. Using C. parapsilosis cells immobilized on AFM cantilevers with a silicone substrate, we have measured attractive forces of 4.3 +/- 0.25 nN in the approach portion of the force cycle. On P. aeruginosa biofilms, the magnitude of the attractive force decreases to 2.0 +/- 0.40 nN and is preceded by a 2.0-nN repulsion at approximately 75 nm from the cell surface. These data suggest that C. parapsilosis may adhere to both silicone rubber and P. aeruginosa biofilms, possibly contributing to patient morbidity and mortality. Characterization of cell-biomaterial and cell-cell interactions allows for a quantitative link between the physicomechanical and physicochemical properties of implant materials and the nanoscale interactions leading to microbial colonization and infection.