Spontaneous Calcium Transients in Cultured Cortical Networks During Development
Spontaneous Calcium Transients in Cultured Cortical Networks During Development
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DOI：
10.1109/tbme.2009.2028419
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发表时间：
2009-08
影响因子：
4.6
通讯作者：
Y. Takayama;H. Moriguchi;K. Kotani;Y. Jimbo
中科院分区：
文献类型：
--
作者：
Y. Takayama;H. Moriguchi;K. Kotani;Y. Jimbo
自发神经元活动在大脑发育中起着重要作用。利用微电极阵列（MEA）记录系统对离体培养的神经元网络自发活动模式的发育变化进行了广泛的研究。然而，很少有人知道自发的细胞内钙动力学的转变，以及钙瞬变和电活动之间的关系在发展。在本文中，我们进行了同时记录自发电活动和细胞内钙瞬变的大鼠皮层网络上MEA培养。在一周的培养中，观察到周期性同步爆发，随后是同步钙瞬变。在三周的文化，同步钙瞬变很少观察到，尽管存在高度复杂的同步活动。在这两种状态之间，在两周的培养物中，观察到钙波的缓慢、径向传播，而不依赖于电活动。嘌呤能受体拮抗剂苏拉明和间隙连接阻断剂18-β-大黄酸的药理学治疗表明，自发放射状钙波是由星形胶质细胞网络介导的，并表明星形胶质细胞钙波可以通过局部影响神经元信号传导来影响网络的电放电模式。这些结果表明，细胞内钙瞬变的各种动力学调节网络的成熟过程。
Spontaneous neuronal activity plays an important role in the development of the brain. Developmental changes in the spontaneous activity pattern of neuronal networks in vitro have been extensively studied by using the microelectrode array (MEA) recording system. However, little is known about the transition of spontaneous intracellular calcium dynamics, and the relationship between calcium transients and electrical activity during development. In the present paper, we carry out simultaneous recording of spontaneous electrical activity and intracellular calcium transients of rat cortical networks cultured on MEA. In one-week cultures, periodic synchronized bursts are observed and are followed by synchronized calcium transients. In three-week cultures, synchronized calcium transients are rarely observed despite the presence of highly complicated synchronized activity. Between these two states, in two-week cultures, slow, radial propagation of calcium waves independent of electrical activity is observed. Pharmacological treatments with the purinergic receptor antagonist suramin and gap junction blocker 18-beta glycyrrhetinic acid reveal that the spontaneous radial calcium waves are mediated by the astrocytic network, and suggest that the astrocytic calcium waves can influence the electrical firing patterns of networks by locally affecting neuronal signaling. These results indicate that the various dynamics of intracellular calcium transients regulate the network maturation processes.