ANTIGENICITY OF PURIFIED GLUTARALDEHYDE-TREATED CHOLERA TOXOID ADMINISTERED ORALLY
ANTIGENICITY OF PURIFIED GLUTARALDEHYDE-TREATED CHOLERA TOXOID ADMINISTERED ORALLY
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DOI：
10.1128/iai.21.1.158-162.1978
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发表时间：
1978-01-01
影响因子：
3.1
通讯作者：
BERGQUIST, EJ
中科院分区：
文献类型：
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作者：
LEVINE, MM;HUGHES, TP;BERGQUIST, EJ
在健康志愿者中研究了口服戊二醛处理的霍乱类毒素的抗原性。14名志愿者摄入2或3个2毫克剂量的类毒素和盐水，剂量间隔为28天。另外13名志愿者接受了类似剂量的NaHCO 3和牛奶来中和胃酸。循环抗毒素水平的增加用于测定口服类毒素的抗原性。分别于免疫后0、28、35、56、63和84天采集血清，用肾上腺细胞法、家兔皮肤通透性因子法和被动血凝试验检测血清抗毒素水平。肾上腺细胞法和家兔皮肤法检测27例免疫者抗毒素升高的敏感性相同（19/27），显著高于被动血凝法（11/27）（P < 0.03）。志愿者谁摄入类毒素与碳酸氢钠和牛奶有较高的血清转换率（77%）比那些谁收到类毒素与盐水（64%），他们也有早期上升的抗毒素滴度和一贯较高的几何平均滴度在所有测试天。经纯化的霍乱类毒素在口服给药后在人体内具有抗原性。
The antigenicity of orally administered glutaraldehyde-treated cholera toxoid was investigated in healthy volunteers. Fourteen volunteers ingested 2 or 3 2-mg doses of toxoid with saline, with the doses spaced at 28-day intervals. Thirteen other volunteers received comparable toxoid doses with NaHCO3 and milk to neutralize gastric acid. Increments in circulating antitoxin levels were used to assay the antigenicity of oral toxoid. Antitoxin was measured by adrenal cell, rabbit skin permeability factor and passive hemagglutination assays in sera collected on days 0, 28, 35, 56, 63 and 84 after primary immunization. Adrenal cell and rabbit skin assays exhibited identical sensitivity in detecting antitoxin rises in the 27 vaccinees (19/27) and were significantly more sensitive than passive hemagglutination (11/27) (P < 0.03). Volunteers who ingested toxoid with NaHCO3 and milk had a higher rate of seroconversion (77%) than those who received toxoid with saline (64%); they also had earlier rises in antitoxin titer and consistently higher geometric mean titers on all days tested. Purified cholera toxoid is antigenic in humans after oral administration.