The genomic diversity of Chinese porcine reproductive and respiratory syndrome virus isolates from 1996 to 2009
The genomic diversity of Chinese porcine reproductive and respiratory syndrome virus isolates from 1996 to 2009
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1996-2009年中国猪繁殖与呼吸综合征病毒基因组多样性
DOI：
10.1016/j.vetmic.2010.05.011
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发表时间：
2010-12-15
影响因子：
3.3
通讯作者：
Xiao, Shaobo
中科院分区：
文献类型：
--
作者：
Li, Bin;Fang, Liurong;Xiao, Shaobo
自1995年首次报道以来，猪繁殖与呼吸综合征（PRRS）已成为中国最重要的猪病之一。在中国不同地区、不同时间分离到了大量的PRRSV野外菌株，特别是在2006年出现高致病性PRRSV之后。以往基于ORF5基因序列的研究显示，中国PRRSV分离株具有广泛的遗传多样性。为了充分了解PRRSV在中国的遗传多样性程度，我们测定了2006年底分离到的一种高致病性PRRSV WUH1的基因组序列。基于1996 - 2009年分离的WUH1株和其他66株中国PRRSV野外菌株的全基因组序列，进一步分析了它们的遗传多样性。结果表明，所有中国PRRSV分离株均属于北美基因型，可明确分为4个高度分化的亚基因型。此外，分析结果支持中国高致病性PRRSV是由中国国内病毒逐渐变异和进化而来的观点。此外，在中国PRRSV分离株中广泛观察到Nsp2的不同缺失、GP3的缺失和潜在的抗原漂移以及GP5的点突变。有趣的是，除了Nsp2中独特的30aa不连续缺失外，几乎所有高致病性PRRSV分离株的5'UTR和3'UTR中都有1个核苷酸缺失。这些结果将有助于阐明PRRSV在中国的进化机制。(C) 2010 Elsevier B.V.版权所有
Since it was first reported in 1995, porcine reproductive and respiratory syndrome (PRRS) has become one of the most important swine diseases in China. A large number of field PRRSV strains have been isolated from different regions of China at different times, especially after a highly pathogenic PRRSV emerged in 2006. Previous studies based on ORF5 gene sequences revealed extensive genetic diversity among Chinese PRRSV isolates. To fully understand the extent of genetic diversity of PRRSV in China, we determined the genomic sequence of PRRSV WUH1, a highly pathogenic PRRSV isolated in late 2006. Based on the complete genomic sequences of strain WUH1 and 66 other field Chinese PRRSV strains isolated from 1996 to 2009, we further analyzed their genetic diversity. The results showed that all the tested Chinese PRRSV isolates belong to the North American genotype and can be clearly divided into four highly diverse subgenotypes. Furthermore, the analysis supported the concept that the highly pathogenic PRRSV in China emerged by gradual variation and evolution from the Chinese domestic virus. In addition, different deletions within Nsp2, deletion and potential antigenic drift within GP3, and point mutations within GP5, were extensively observed in Chinese PRRSV isolates. Interestingly, in addition to a unique discontinuous deletion of 30 aa in Nsp2, there was a 1 nucleotide deletion in both the 5'UTR and 3'UTR in nearly all highly pathogenic PRRSV isolates. These results will contribute to the elucidation of the evolutionary mechanisms of PRRSV in China. (C) 2010 Elsevier B.V. All rights reserved.