Characterization of skin cytokines in bullous pemphigoid and pemphigus vulgaris
Characterization of skin cytokines in bullous pemphigoid and pemphigus vulgaris
复制标题
大疱性类天疱疮和寻常型天疱疮皮肤细胞因子的特征
作者：
Rico;Benning;Weingart;Streilein;Hall
本研究的目的是检测大疱性类天疱疮(BP，n = 21)和寻常型天疱疮(PV，n = 7)患者皮损周围皮肤活检组织中细胞因子和细胞标志物的表达。免疫组织化学和原位杂交法检测辅助性T细胞(Th)1[IL-2、干扰素-γ]和Th2(IL-4、IL-5、IL-13)蛋白和基因表达。BP患者皮损组织中IL-4、IL-13和IL-5的沉积以IL-4、IL-13和IL-5的沉积为特征。在BP患者中，IL-4和IL-13定位于真皮浸润区内的单个核细胞，而IL-5主要表达于真皮-表皮交界处。BP皮肤切片也表达血管细胞黏附分子1，在PV患者中未见。PV组织中存在IL-2、干扰素-γ和IL-4，而不表达IL-5和IL-13。原位杂交检测到BP患者细胞浸润液中IL-4和IL-5mRNA的表达，1例PV患者细胞中IL-2mRNA的表达。体外结合分析表明，经IL-5共培养激活的正常人嗜酸性粒细胞优先与含有体内可检测到的IL-5结合的BP皮肤切片结合。BP中Th2细胞因子的优势与Eos体外结合的增加相结合，提示Th2细胞因子与BP患者真皮浸润液中Eos的募集和黏附有关，并可能在水泡形成的发病机制中发挥作用。
The purpose of this study was to determine cytokine and cell marker expression in perilesional skin biopsies from patients with the autoimmune blistering diseases bullous pemphigoid (BP, n = 21) and pemphigus vulgaris (PV, n = 7). Immunohistochemistry and in situ hybridization were used to detect T helper (Th)1 [interleukin (IL)‐2, interferon (IFN)‐γ] and Th2 (IL‐4, IL‐5, IL‐13) protein and mRNA. Perilesional skin biopsies from patients with BP were characterized by the deposition of IL‐4, IL‐13 and IL‐5. In patients with BP, IL‐4 and IL‐13 localized to mononuclear cells within the dermal infiltrate while IL‐5 was predominately expressed at the dermal–epidermal junction. BP skin sections also expressed vascular cell adhesion molecule 1 on endothelial cells, not seen in patients with PV. PV biopsies were remarkable for a mixed Th1/Th2 pattern of cytokine expression, including the presence of IL‐2, IFN‐γ and IL‐4 and the absence of IL‐5 and IL‐13. In situ hybridization detected mRNA for IL‐4 and IL‐5 in the cellular infiltrate of BP patients, and IL‐2 in a patient with PV. In vitro binding assays demonstrated that normal human eosinophils, activated by coculture in IL‐5, bound preferentially to BP skin sections that contained detectable in vivo bound IL‐5. The predominance of Th2 cytokines in BP, in association with increased binding of eosinophils in vitro, suggests that Th2 cytokines are relevant in the recruitment and adhesion of eosinophils within the dermal infiltrates of patients with BP, and may play a part in the pathogenesis of blister formation.
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影响因子：
2.2
作者：
T. Taguchi;J. Mcghee;R. Coffman;K. Beagley;J. Eldridge;K. Takatsu;H. Kiyono
通讯作者：
T. Taguchi;J. Mcghee;R. Coffman;K. Beagley;J. Eldridge;K. Takatsu;H. Kiyono
DOI：
10.1073/pnas.92.25.11935
发表时间：
1995-12
影响因子：
11.1
作者：
Kai W. WUCHERPFENNIGt;Bei Yut;Kailash BHOLt;Dimitri S. Monos;Elias Argyris;W. Robert;Karr;A. R. AHMEDt;J. L. STROMINGERt
通讯作者：
Kai W. WUCHERPFENNIGt;Bei Yut;Kailash BHOLt;Dimitri S. Monos;Elias Argyris;W. Robert;Karr;A. R. AHMEDt;J. L. STROMINGERt
影响因子：
15.9
作者：
STAHLEBACKDAHL, M;INOUE, M;PARKS, WC
通讯作者：
PARKS, WC
影响因子：
4.4
作者：
R. Schleimer;S. A. Sterbinsky;James Kaiser;C. Bickel;David Klunk;K. Tomioka;W. Newman;F. Luscinskas;M. Gimbrone;B. McIntyre
通讯作者：
R. Schleimer;S. A. Sterbinsky;James Kaiser;C. Bickel;David Klunk;K. Tomioka;W. Newman;F. Luscinskas;M. Gimbrone;B. McIntyre
影响因子：
15.9
作者：
Lin, MS;Swartz, SJ;Diaz, LA
通讯作者：
Diaz, LA