Inhibition of touch cell fate by egl-44 and egl-46 in C. elegans.
Inhibition of touch cell fate by egl-44 and egl-46 in C. elegans.
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DOI：
10.1101/gad.857401
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发表时间：
2001-03
影响因子：
10.5
通讯作者：
Ji Wu;Anne Duggan;Anne Duggan;M. Chalfie
中科院分区：
文献类型：
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作者：
Ji Wu;Anne Duggan;Anne Duggan;M. Chalfie
在野生型秀丽隐杆线虫中，有六个细胞发育为温柔触摸的受体。在egl-44和egl-46突变体中，另外两个神经元，FLP细胞，表达触摸受体样特征。egl-44和egl-46还影响其它神经元的分化，包括HSN神经元，这是产卵所需的两种细胞。EGL-44编码转录增强因子家族的一个成员。egl-46基因的产物、两种果蝇蛋白以及人和小鼠中的两种蛋白定义了一个新的锌指蛋白家族。egl-44和egl-46都在FLP和HSN神经元（和其他细胞）中表达; egl-46的表达在FLP细胞中依赖于egl-44，但在HSN细胞中不依赖。野生型触觉细胞表达egl-46但不表达egl-44。此外，触摸细胞中egl-44的异位表达以egl-46依赖的方式阻止触摸细胞分化。这些基因的序列和它们在GFP融合体中的核定位表明它们抑制FLP细胞中触觉细胞特征的转录。
In wild-type Caenorhabditis elegans, six cells develop as receptors for gentle touch. In egl-44 and egl-46 mutants, two other neurons, the FLP cells, express touch receptor-like features. egl-44 and egl-46 also affect the differentiation of other neurons including the HSN neurons, two cells needed for egg laying. egl-44 encodes a member of the transcription enhancer factor family. The product of the egl-46 gene, two Drosophila proteins, and two proteins in human and mice define a new family of zinc finger proteins. Both egl-44 and egl-46 are expressed in FLP and HSN neurons (and other cells); expression of egl-46 is dependent on egl-44 in the FLP cells but not in the HSN cells. Wild-type touch cells express egl-46 but not egl-44. Moreover, ectopic expression of egl-44 in the touch cells prevents touch cell differentiation in an egl-46-dependent manner. The sequences of these genes and their nuclear location as seen with GFP fusions indicate that they repress transcription of touch cell characteristics in the FLP cells.