Poly (D,L-lactide-co-glycolide) nanoparticle-entrapped vaccine induces a protective immune response against porcine epidemic diarrhea virus infection in piglets
Poly (D,L-lactide-co-glycolide) nanoparticle-entrapped vaccine induces a protective immune response against porcine epidemic diarrhea virus infection in piglets
复制标题
聚（d,l-丙交酯-乙交酯）纳米颗粒包埋疫苗可诱导仔猪产生针对猪流行性腹泻病毒感染的保护性免疫反应。
DOI：
10.1016/j.vaccine.2017.10.054
复制
发表时间：
2017-12-15
期刊：
影响因子：
5.5
通讯作者：
He, Kongwang
中科院分区：
文献类型：
--
作者：
Li, Bin;Du, Luping;He, Kongwang
猪流行性腹泻（PED）是一种严重危害养猪业的疾病，其病原体猪流行性腹泻病毒（PEDV）对新生仔猪的死亡率高达80-100%。本研究制备了可生物降解的聚（D，L-丙交酯-共-乙交酯）（PLGA）纳米颗粒包埋PEDV灭活疫苗抗原（KAg）（PLGA-KAg）。用PLGA-KAg鼻内接种晚期妊娠母猪，并在其被动免疫的哺乳仔猪中研究由高毒力PEDV攻击引起的死亡率。PEDV特异性IgG和伊加抗体滴度在PLGA-ICAg免疫的妊娠母猪中相对于接种KAg的母猪中的滴度增强。在这些母猪的被动免疫哺乳仔猪中观察到类似的结果。与接种KAg或Montanid（TM）伊萨201 VG乳化灭活PEDV疫苗（201-KAg）的妊娠母猪相比，在用PLGA-KAg免疫的妊娠母猪中诱导了改善的淋巴细胞增殖应答和IFN-γ水平。重要的是，接种PLGA-KAg的组中仔猪死亡率低于接种KAg或201-KAg的组。这些结果表明PLGA-KAg是一种有希望的候选疫苗，可以在哺乳仔猪中提供针对PEDV感染的保护性免疫。(C)2017爱思唯尔有限公司版权所有
Porcine epidemic diarrhea (PED) causes 80-100% mortality in neonatal piglets, and its causative agent, the porcine epidemic diarrhea virus (PEDV), poses an important threat to the swine industry worldwide. In this study, we prepared biodegradable poly (D,L-lactide-co-glycolide) (PLGA) nanoparticle-entrapped PEDV killed vaccine antigens (KAg) (PLGA-KAg). Late-term pregnant sows were intranasally inoculated with PLGA-KAg, and the mortality resulting from challenge with highly virulent PEDV was investigated in their passively immunized suckling piglets. PEDV-specific IgG and IgA antibody titers were enhanced in pregnant sows immunized with PLGA-ICAg relative to the titers in sows inoculated with KAg. Similar results were seen in the passively immunized suckling piglets of these sows. Improved lymphocyte proliferation responses and IFN-gamma levels were induced in pregnant sows immunized with PLGA-KAg compared with those vaccinated with KAg or with Montanid (TM) ISA 201 VG emulsified killed PEDV vaccine (201-KAg). Importantly, there was less piglet mortality in the group vaccinated with PLGA-KAg than in the groups vaccinated with KAg or 201-KAg. These results demonstrate that PLGA-KAg is a promising candidate vaccine that can provide protective immunity against PEDV infection in suckling piglets. (C) 2017 Elsevier Ltd. All rights reserved.