ISOLATION AND CHARACTERIZATION OF SFP - A GENE THAT FUNCTIONS IN THE PRODUCTION OF THE LIPOPEPTIDE BIOSURFACTANT, SURFACTIN, IN BACILLUS-SUBTILIS
ISOLATION AND CHARACTERIZATION OF SFP - A GENE THAT FUNCTIONS IN THE PRODUCTION OF THE LIPOPEPTIDE BIOSURFACTANT, SURFACTIN, IN BACILLUS-SUBTILIS
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DOI：
10.1007/bf00280011
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发表时间：
1992-03-01
期刊：
影响因子：
--
通讯作者：
ZUBER, P
中科院分区：
文献类型：
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作者：
NAKANO, MM;CORBELL, N;ZUBER, P
SFP基因是枯草芽孢杆菌细胞产生脂肽抗生素表面蛋白所必需的。分离SFP并测定其核苷酸序列。在大肠杆菌中表达了SFP，并对其产物进行了纯化，用于抗体生产和氨基酸序列分析。该基因在枯草杆菌中进行了质粒扩增，使sfp0(不产生表面蛋白)细胞具有SRF+表型。枯草杆菌中SFP的过量生产并没有导致表面素量的增加，而是导致了srfA操纵子的LacZ转录融合的抑制，srfA操纵子编码催化表面素合成的酶。我们认为SFP是多肽合成系统的重要组成部分，在表面蛋白生物合成基因表达的调控中起着直接或间接的作用。
The sfp gene is required for cells of Bacillus subtilis to become producers of the lipopeptide antibiotic surfactin. sfp was isolated and its nucleotide sequence was determined. sfp was expressed in Escherichia coli and its putative product was purified for use in antibody production and in amino acid sequence analysis. The gene was plasmid-amplified in B. subtilis, where it conferred a Srf+ phenotype on sfp0 (surfactin non-producing) cells. Overproduction of Sfp in B. subtilis did not cause production of an increased amount of surfactin and resulted in the repression of a lacZ transcriptional fusion of the srfA operon, which encodes enzymes that catalyze surfactin synthesis. We propose that sfp represents an essential component of peptide synthesis systems and also plays a role, either directly or indirectly, in the regulation of surfactin biosynthesis gene expression.