Autotrophic denitrification of nitrate rich wastewater in fluidized bed reactors using pyrite and elemental sulfur as electron donors
Autotrophic denitrification of nitrate rich wastewater in fluidized bed reactors using pyrite and elemental sulfur as electron donors
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DOI：
10.1016/j.eti.2022.102878
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发表时间：
2022-08-29
影响因子：
7.1
通讯作者：
Lens, Piet N. L.
中科院分区：
文献类型：
--
作者：
Carboni, Maria F.;Mills, Simon;Lens, Piet N. L.
以元素硫(S0)和黄铁矿(FeS2)为电子供体，比较了以元素硫(S0)和黄铁矿(FeS2)为电子供体的自养反硝化床反应器(FBR)中的反硝化性能和微生物群落。反应器运行220d，硝酸盐负荷率为23~200mgN-NO-3/L，水力停留时间为48~4h，最高反硝化速率分别为142.2 mg NNO-3/L和184.4 mg N-3/L。黄铁矿驱动的反硝化作用产生的SO2-4较少，不需要添加缓冲剂来调节pH。硫磺FBR需要CaCO3来维持pH中性，因此产生了更多的污泥(CaSO4沉淀)。对黄铁矿系统的活性群落进行了调查，发现固氮螺菌属、铁基杆菌属、红球菌属。和假单胞菌属(Pseudomonas sp.)为优势属，硫杆菌属为优势种。和Sulfurovum sp.主导了硫磺快堆中活跃的群落。而硫杆菌属(Thibacussp.)在较高的氮气负荷率下运行时变得更具优势。多样性和微生物群落的格局分析表明，微生物群落演替经历了三个不同的阶段，这与进水硝酸盐浓度较高的时期(135mgN-NO-3/L)的运行相对应。有人提出，初始微生物群落的高度功能冗余可能有助于两个反应器对如此高的进水硝酸盐浓度做出更好的响应。(C)2022年作者(S)。由Elsevier B.V.出版。这是CC BY-NC-ND许可证(http://creativecommons.org/licenses/by-nc-nd/4.0/).下的一篇开放获取文章
This study compared denitrification performances and microbial communities in fluidized bed reactors (FBRs) carrying out autotrophic denitrification using elemental sulfur (S0) and pyrite (FeS2) as electron donors. The reactors were operated for 220 days with nitrate loading rates varying between 23 and 200 mg N-NO-3 /Lmiddotd and HRT between 48 and 4 h. The highest denitrification rates achieved were 142.2 and 184.4 mg NNO-3 /Lmiddotd in pyrite and sulfur FBRs, respectively. Pyrite-driven denitrification produced less SO2- 4 and no buffer addition was needed to regulate the pH. The sulfur FBR needed instead CaCO3 to maintain the pH neutral and consequentially more sludge was produced (CaSO4 precipitation). The active community of pyrite-based systems was investigated and Azospira sp., Ferruginibacter sp., Rhodococcus sp. and Pseudomonas sp. were the predominant genera, while Thiobacillus sp. and Sulfurovum sp. dominated the active community in the sulfur FBR. However, Thiobacillus sp. became more dominant when operating at elevated nitrogen loading rate. Patterns of diversity and microbial community assembly were assessed and revealed three distinct stages of microbial community succession which corresponded with the operation of a period of high influent nitrate concentration (135 mg N-NO-3 /L). It is proposed that a high degree of functional redundancy in the initial microbial communities may have helped both reactors to respond better to such high influent nitrate concentration.(c) 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).