Differential Expression Profiles of Circular RNAs During Osteogenic Differentiation of Mouse Adipose-Derived Stromal Cells
Differential Expression Profiles of Circular RNAs During Osteogenic Differentiation of Mouse Adipose-Derived Stromal Cells
复制标题
小鼠脂肪基质细胞成骨分化过程中环状 RNA 的差异表达谱
DOI：
10.1007/s00223-018-0426-0
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发表时间：
2018-09-01
影响因子：
4.2
通讯作者：
Long, Jie
中科院分区：
文献类型：
--
作者：
Long, Ting;Guo, Zeyou;Long, Jie
成骨是一个复杂且严格调控的过程。环状RNA（circRNA）是共价闭合的RNA分子，被认为在骨代谢中发挥重要作用。本研究的目的是研究小鼠脂肪源性基质细胞 (mADSC) 成骨分化过程中 circRNA 的表达和假定功能。 circRNA 微阵列用于测定 mADSC 成骨过程中不同阶段的差异 circRNA 表达。通过维恩分析选择最常见的差异表达的 circRNA，并将其聚集在三个诱导组中。此外，还利用生物信息学分析（基因本体、通路和共表达网络分析）来进一步研究这些差异表达的circRNA。总共检测到 14,236 个 circRNA，其中 43 个 circRNA（40 个上调）在 mADSC 成骨分化过程中的指定时间点持续改变。与其他类型的 circRNA 相比，外显子 circRNA 在差异表达的 circRNA 中所占的比例明显更大。通过基因本体论和京都基因与基因组百科全书生物学途径分析来评估差异表达的circRNA在成骨过程中的功能。我们的 circRNA-miRNA 共表达网络表明 miR-338-3p 与两个 circRNA（mmu_circRNA_013422、mmu_circRNA_22566）的上调相关。我们关于 circRNA 表达谱的数据可能为了解 mADSC 成骨分化过程中 circRNA 的功能提供有价值的见解。此外，circRNA-miRNA-mRNA 通路可能为骨形成和再生的临床研究提供新机制和靶点的信息。
Osteogenesis is a complex and tightly regulated process. Circular RNAs (circRNAs) are covalently closed RNA molecules which are thought to play a significant role in bone metabolism. The purpose of this study was to investigate the expression and putative function of circRNAs during the osteogenic differentiation of mouse adipose-derived stromal cells (mADSCs). circRNA microarrays were used to determine differential circRNAs expression at different stages during osteogenesis of mADSCs. The most frequent differentially expressed circRNAs were selected by Venn analysis and clustered among the three induced groups. In addition, bioinformatic analyses (gene ontology, pathway, and co-expression network analysis) were used to further investigate these differentially expressed circRNAs. A total of 14,236 circRNAs were detected, of which 43 circRNAs (40 upregulated) were consistently altered at indicated time points during osteogenic differentiation of mADSCs. The exonic circRNAs represented a significantly larger proportion among the differentially expressed circRNAs compared to other types of circRNAs. Gene ontology and Kyoto Encyclopedia of Genes and Genomes biological pathway analysis were performed to evaluate the functions of differentially expressed circRNAs during the osteogenic process. Our circRNA–miRNA co-expression network showed that miR-338-3p was correlated with upregulation of two circRNAs (mmu_circRNA_013422, mmu_circRNA_22566). Our data on circRNA expression profiles may provide valuable insight into circRNA function during osteogenic differentiation of mADSCs. Additionally, the circRNA–miRNA–mRNA pathways may provide information on novel mechanisms and targets for clinical investigations on bone formation and regeneration.