Use of stem cell markers in dissociated mammary populations.
Use of stem cell markers in dissociated mammary populations.
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在分离的乳腺群体中使用干细胞标记。
DOI：
10.1007/978-1-60761-063-2_3
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Welm,BryanE
中科院分区：
文献类型：
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作者：
Shelton,DawneN;Fernandez-Gonzalez,Rodrigo;Illa-Bochaca,Irineu;Ortiz-de-Solorzano,Carlos;Barcellos-Hoff,MaryHelen;Welm,BryanE
乳腺上皮的再生潜能有助于在移植试验中评估任何上皮亚群的“干性”。因此，乳腺组织可以分离成单个细胞，染色获得感兴趣的细胞表面标记物，并使用荧光激活的细胞分选进行分类。然后可以将选定的细胞移植到来自受体宿主的分离上皮的脂肪垫中。最近的出版物描述了丰富乳腺再生潜力的标记。在这里，我们描述了根据这些标记分选细胞所需的材料和方法。这种方法可以与其他细胞表面标记物互换使用，对协议有轻微的变化。
The regenerative potential of mammary epithelium facilitates assessment of the “stemness” of any epithelial subpopulation in transplantation assays. Thus, mammary tissue can be dissociated into single cells, stained for cell surface markers of interest and classified using fluorescence-activated cell sorting. The selected cells can then be transplanted into epithelium-devoided fat pads from recipient hosts. Recent publications have described markers that enrich for mammary repopulating potential. Here, we describe the materials and methods necessary to sort cells according to these markers. This approach can be used interchangeably with other cell surface markers with slight variation to the protocol.