Imaging neuronal migration and network activity in human forebrain assembloids.
Imaging neuronal migration and network activity in human forebrain assembloids.
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DOI：
10.1016/j.xpro.2022.101478
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发表时间：
2022-09-16
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--
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中科院分区：
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由人类多能干细胞产生的类组装体是自组织的多细胞体外模型，其概括了神经发育期间细胞-细胞相互作用和电路组装的方面。在这里，我们提出的协议，功能监测，在前脑类胶质细胞，GABA能中间神经元从腹侧到背侧前脑和早期皮质网络的活动迁移。具体来说，我们描述了高分辨率成像和分析神经元迁移以及钙成像的网络活动在前脑类胶质细胞。有关本方案使用和执行的完整详细信息，请参阅。前脑类胶质细胞模型迁移和皮质回路形成在类胶质细胞中以高分辨率成像人类皮质迁移用钙成像探测类胶质细胞中神经网络的形成出版商注：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指南。由人类多能干细胞产生的类组装体是自组织的多细胞体外模型，其概括了神经发育期间细胞-细胞相互作用和电路组装的方面。在这里，我们提出的协议，功能监测，在前脑类胶质细胞，GABA能中间神经元从腹侧到背侧前脑和早期皮质网络的活动迁移。具体来说，我们描述了高分辨率成像和分析神经元迁移以及钙成像的网络活动在前脑类胶质细胞。
Assembloids generated from human pluripotent stem cells are self-organizing, multicellular in vitro models that recapitulate aspects of cell-cell interactions and circuit assembly during neural development. Here, we present protocols to functionally monitor, in forebrain assembloids, the migration of GABAergic interneurons from the ventral to the dorsal forebrain and the activity in early cortical networks. Specifically, we describe high-resolution imaging and analysis of neuronal migration as well as calcium imaging of network activity in forebrain assembloids. For complete details on the use and execution of this protocol, please refer to. Forebrain assembloids model migration and cortical circuit formation Image human cortical migration at high resolution in assembloids Probe the formation of neural networks in assembloids with calcium imaging Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Assembloids generated from human pluripotent stem cells are self-organizing, multicellular in vitro models that recapitulate aspects of cell-cell interactions and circuit assembly during neural development. Here, we present protocols to functionally monitor, in forebrain assembloids, the migration of GABAergic interneurons from the ventral to the dorsal forebrain and the activity in early cortical networks. Specifically, we describe high-resolution imaging and analysis of neuronal migration as well as calcium imaging of network activity in forebrain assembloids.
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