Comparative proteomics of hydatid fluids from two Echinococcus multilocularis isolates
Comparative proteomics of hydatid fluids from two Echinococcus multilocularis isolates
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DOI：
10.1016/j.jprot.2017.04.009
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发表时间：
2017-06-06
影响因子：
3.3
通讯作者：
Ferreira, Henrique B.
中科院分区：
文献类型：
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作者：
Monteiro, Karina M.;Lorenzatto, Karina R.;Ferreira, Henrique B.
充满多房棘球绦虫囊泡的包虫液 (HF) 是来自寄生虫和宿主的蛋白质的复杂混合物。在此，使用 LC-MS/MS 方法比较多房艾美耳球虫 H95 和 G8065 分离株（分别为 EmH95 和 EmG8065）的 HF 组成，它们在生长和繁殖力方面存在差异。总体而言，鉴定出 446 种独特蛋白质，其中 392 种 (88%) 来自寄生虫，54 种来自培养基。至少 256 种寄生虫蛋白是样本独有的，136 种共享蛋白中的 82 种在多房艾美耳球菌分离株之间呈现丰度差异。 EmH95 和 EmG8065 HF 样本中寄生虫的蛋白质谱呈现出涉及抗原、信号蛋白、蛋白水解酶、蛋白酶抑制剂和分子伴侣的定性和定量差异，突出了可能与每个分离株的生物学特征相关的种内奇异性。 HF 中发现的培养基蛋白质库在分离株之间也存在差异，并且讨论了 HF 外源蛋白质含量与寄生虫生物学的相关性。已鉴定的蛋白质库还为重要的生物学特征（例如寄生虫生长速度和繁殖力）提供了潜在的分子标记，以及开发肺泡包虫病新型诊断和治疗策略的潜在蛋白质靶点。生物学意义：哺乳动物宿主的多房棘球蚴感染涉及由排泄/分泌（ES）产物介导的复杂相互作用。充满多房棘球绦虫囊泡的包虫液 (HF) 含有复杂的寄生虫 ES 产物和宿主蛋白，介导重要的分子相互作用，决定寄生虫的生存和发育，从而影响感染结果。 HF 也被广泛报道为包虫病免疫诊断的主要蛋白质来源。所进行的蛋白质组分析提供了两种多房艾美耳球虫分离株的 HF 蛋白质组成的全面分析。这使我们能够识别寄生虫和外源（中等）来源的蛋白质，其中许多蛋白质在寄生虫分离株之间呈现显着的丰度差异，并可能与其不同的生物学特征（包括生育力和生长速度）相关。 (C) 2017 Elsevier B.V. 保留所有权利。
The hydatid fluid (HF) that fills Echinococcus multilocularis metacestode vesicles is a complex mixture of proteins from both parasite and host origin. Here, a LC-MS/MS approach was used to compare the HF composition of E. multilocularis H95 and G8065 isolates (EmH95 and EmG8065, respectively), which present differences in terms of growth and fertility. Overall, 446 unique proteins were identified, 392 of which (88%) were from parasite origin and 54 from culture medium. At least 256 of parasite proteins were sample exclusive, and 82 of the 136 shared proteins presented differential abundance between E. multilocularis isolates. The parasite's protein repertoires in EmH95 and EmG8065 HF samples presented qualitative and quantitative differences involving antigens, signaling proteins, proteolytic enzymes, protease inhibitors and chaperones, highlighting intraspecific singularities that could be correlated to biological features of each isolate. The repertoire of medium proteins found in the HF was also differential between isolates, and the relevance of the HF exogenous protein content for the parasite's biology is discussed. The repertoires of identified proteins also provided potential molecular markers for important biological features, such as parasite growth rate and fertility, as well potential protein targets for the development of novel diagnostic and treatment strategies for alveolar echinococcosis.Biological significance: E. multilocularis metacestode infection of mammal hosts involve complex interactions mediated by excretory/secretory (ES) products. The hydatid fluid (HF) that fills the E. multilocularis metacestode vesicles contains complex repertoires of parasite ES products and host proteins that mediate important molecular interactions determinant for parasite survival and development, and, consequently, to the infection outcome. HF has been also extensively reported as the main source of proteins for the immunodiagnosis of echinococcosis. The performed proteomic analysis provided a comprehensive profiling of the HF protein composition of two E. multilocularis isolates. This allowed us to identify proteins of both parasite and exogenous (medium) origin, many of which present significant differential abundances between parasite isolates and may correlate to their differential biological features, including fertility and growth rate. (C) 2017 Elsevier B.V. All rights reserved.