PROBING CANCER SIGNALING WITH RESONANT WAVEGUIDE GRATING BIOSENSORS.
PROBING CANCER SIGNALING WITH RESONANT WAVEGUIDE GRATING BIOSENSORS.
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DOI：
10.1517/17460441.2010.533652
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发表时间：
2010-12
影响因子：
6.3
通讯作者：
Fang Y
中科院分区：
文献类型：
--
作者：
Fang Y
癌症是由体细胞遗传改变的逐渐积累引起的一系列疾病。基因组学方法已经确定了与肿瘤发生相关的多种遗传异常，分子成像和定量分析进一步阐明了途径内部或途径之间的复杂相互作用。众所周知，是蛋白质而不是基因完成了大多数细胞功能；信号蛋白在很大程度上是通过一个庞大而复杂的网络运作的。因此，癌症主要是一种通路失调的疾病——少数核心通路在导致癌症的异常细胞生长中占主导地位。因此，在原生信号网络的背景下，了解信号蛋白失调和/或突变的功能后果是癌症研究的前沿。本文综述了为什么谐振波导光栅（RWG）生物传感器细胞检测被认为是综合性质的，以及RWG生物传感器如何用于挖掘癌细胞表面标记物，以及发现癌症受体信号传导的核心途径。读者将从途径的角度获得癌症生物学的概述，并对RWG生物传感器在癌症研究和诊断中所承诺的综合细胞测定的潜在含义有一个粗略的了解。开发下一代抗癌疗法和诊断方案的成功方法应该考虑到致癌途径的失调是肿瘤发生的核心。生物传感器细胞检测在表征癌症生物学方面提供了前所未有的优势。然而，在反卷积和验证使用这些检测在癌症受体信号中确定的细胞机制方面也提出了重大挑战。
Cancer is a collection of diseases that arise from the progressive accumulation of genetic alterations in somatic cells. Genomic approaches have identified a great variety of genetic abnormalities associated with tumorigenesis, and molecular imaging and quantification assays have further elucidated the complex interactions within or between pathways. It is acknowledged that it is proteins, rather than genes, to fulfill most cellular functions; and signaling proteins largely operate through a large and complex network. To this end, cancer is mostly a pathway dysregulated disease – a small number of core pathways are dominate in aberrant cell growth leading to cancer. Thus, understanding the functional consequences of dysregulated and/or mutant signaling proteins in the context of native signaling networks is the frontier in cancer research. This article reviews why resonant waveguide grating (RWG) biosensor cellular assays are considered to be integrative in nature, and how RWG biosensor can be used for mining the surface markers of cancer cells, and discovering core pathway(s) of cancer receptor signaling. The reader will gain an overview of cancer biology from pathway perspective, and have a glimpse of potential implications of integrative cellular assays, as promised by RWG biosensor, in cancer research and diagnosis. Successful approaches for developing next-generation anti-cancer therapies and diagnostic protocols should take into account that the dysregulation of oncogenic pathways is central to tumorigenesis. The biosensor cellular assays offer unprecedented advantage in characterizing cancer biology. However, significant challenges are also presented in deconvoluting and validating cellular mechanisms identified in cancer receptor signaling using these assays.
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影响因子：
15.8
作者：
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发表时间：
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影响因子：
11.1
作者：
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DOI：
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发表时间：
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期刊：
Journal of receptor and signal transduction research
影响因子：
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作者：
Du Y;Li Z;Li L;Chen ZG;Sun SY;Chen P;Shin DM;Khuri FR;Fu H
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Fu H
影响因子：
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作者：
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