Characteristics and Distribution of Extracellular Proteases from Aeromonas hydrophila
Characteristics and Distribution of Extracellular Proteases from Aeromonas hydrophila
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嗜水气单胞菌胞外蛋白酶的特性和分布
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
R. Stevenson
中科院分区：
文献类型：
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作者：
K. Leung;R. Stevenson
摘要：嗜水气单胞菌NRC 505和ATCC 7966的主要胞外蛋白酶分别是耐热金属蛋白酶（TSMP）和耐热丝氨酸蛋白酶（TLSP）。纯化的酶对56°C加热30分钟的敏感性不同，对抑制剂EDTA和苯基甲基磺酰氟（PMSF）有反应，并且抗原性不同。筛选了47株嗜水单胞杆菌的粗细胞外产物（ECP），其中27株同时产生TSMP和TLSP。19株菌株胞外蛋白酶仅产TSMP，而只有嗜水杆菌ATCC 7966胞外蛋白酶唯一产TLSP。这种蛋白酶在菌株之间的分布解释了对单株嗜水单胞杆菌进行检查的研究中相互矛盾的报告。嗜水菌的TLSP与沙门氏菌的蛋白酶相似。sobria和caviae的部分菌株产生TSMP或TLSP。
SUMMARY: The major extracellular proteases of Aeromonas hydrophila NRC 505 and ATCC 7966 are a thermostable metallo-protease (TSMP) and a thermolabile serine-protease (TLSP), respectively. The purified enzymes differed in sensitivity to heating at 56 °C for 30 min, in response to the inhibitors EDTA and phenylmethylsulphonyl fluoride (PMSF), and were antigenically distinct. When crude extracellular products (ECP) of 47 strains of A. hydrophila were screened, 27 strains produced both TSMP and TLSP. TSMP was the only extracellular protease produced by 19 strains, whereas only A. hydrophila ATCC 7966 produced TLSP as its sole protease. This distribution of protease enzymes among strains explains conflicting reports from studies in which single strains of A. hydrophila were examined. The TLSP of A. hydrophila was similar to the protease of A. salmonicida. Either or both TSMP and TLSP were produced by some strains of A. sobria and A. caviae.