Immune responses of goats persistently infected with caprine arthritis-encephalitis virus
Immune responses of goats persistently infected with caprine arthritis-encephalitis virus
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山羊关节炎脑炎病毒持续感染山羊的免疫反应
DOI：
10.1128/iai.28.2.421-427.1980
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发表时间：
1980
影响因子：
3.1
通讯作者：
L. Perryman
中科院分区：
文献类型：
--
作者：
D. S. Adams;T. Crawford;K. Banks;T. McGuire;L. Perryman
将8只剖宫产羔羊接种山羊关节炎脑炎病毒(CAEV)，连续9个月观察其外周血单个核细胞对有丝分裂原和CAEV抗原的增殖反应。用酶联免疫吸附试验检测CAEV特异性抗体。同时检测了5只剖宫产未感染的山羊。感染组和对照组单个核细胞对CAEV的增殖反应在接种后14d开始出现明显差异，并持续波动至接种后134d。感染山羊的增殖反应强度在接种后271天稳定增加，直到实验结束。感染山羊和对照山羊对有丝分裂原的反应没有显著差异。接种病毒的山羊产生CAEV特异性抗体，在接种后49-77天达到最高水平，然后在接种后271天下降到较低水平。接种病毒的山羊出现了轻微但具有特征的山羊关节炎-脑炎的临床证据，并在接种后286天从4只山羊中重新分离到CAEV。5只对照山羊既没有发生抗CAEV免疫反应，也没有出现临床疾病，也不能从它们身上重新分离出CAEV。
Eight cesarean-derived goat kids were inoculated with caprine arthritis-encephalitis virus (CAEV), and proliferative responses of their peripheral blood mononuclear cells to mitogens and CAEV antigen were monitored for 9 months. Antibody specific for CAEV was measured by an enzyme-linked immunosorbent assay. Five cesarean-derived noninfected goats were tested simultaneously. Significant differences between the infected and control mononuclear cell proliferation reactions to CAEV began 14 days post-inoculation and continued in a fluctuant manner until 134 days post-inoculation. The magnitude of the proliferative reaction steadily increased in infected goats until the end of the experiment at 271 days post-inoculation. Responses to mitogens were not significantly different between infected and control goats. Virus-inoculated goats produced CAEV-specific antibody that reached a maximum level between 49 and 77 days post-inoculation and then declined to lower levels through 271 days post-inoculation. The virus-inoculated goats developed mild but characteristic clinical evidence of caprine arthritis-encephalitis, and CAEV was reisolated from four goats at 286 days post-inoculation. The five control goats developed neither an anti-CAEV immune response nor clinical disease, and CAEV could not be reisolated from them.