Presence of adherent Escherichia coli strains in ileal mucosa of patients with Crohn's disease
Presence of adherent Escherichia coli strains in ileal mucosa of patients with Crohn's disease
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DOI：
10.1016/s0016-5085(98)70019-8
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发表时间：
1998-12-01
期刊：
影响因子：
29.4
通讯作者：
Colombel, JF
中科院分区：
文献类型：
--
作者：
Darfeuille-Michaud, A;Neut, C;Colombel, JF
背景和目标：传染性病原体被怀疑参与克罗恩病的发病机制，本研究旨在寻找与克罗恩病患者回肠粘膜相关的毒性大肠杆菌菌株的存在。方法：E.从切除的慢性回肠病变（n = 20）、术后有内镜复发（n = 19）和无内镜复发（n = 11）的患者的新末端回肠以及对照（n = 13）中回收大肠杆菌菌株。使用肠细胞系体外测定细菌粘附;通过DNA杂交和聚合酶链反应实验评估其他相关毒力因子。所有菌株均不携带任何E.大肠埃希菌与急性肠道疾病的关系密切，但对分化的Caco-2细胞的粘附率分别为84.6%和78.9%。慢性和早期复发病灶分离的大肠杆菌菌株分别为33%和33%（P < 0.02）。此外，21.8%的菌株通过合成α-溶血素诱导细胞溶解作用。结论：E.从克罗恩病患者的回肠粘膜分离的大肠杆菌菌株粘附于分化的肠细胞，并可能通过合成α-溶血素破坏肠屏障。
Background & Aims: Infectious agents are suspected of being involved in the pathogenesis of Crohn's disease, This study was designed to look for the presence of virulent Escherichia coli strains associated with the ileal mucosa of patients with Crohn's disease. Methods: E. coli strains were recovered from resected chronic ileal lesions (n = 20), neoterminal ileum after surgery from patients with (n = 19) and without (n = 11) endoscopic recurrence, and controls (n = 13), Bacterial adhesion was determined in vitro using intestinal cell lines; other associated virulence factors were assessed by DNA hybridization and polymerase chain reaction experiments, Results: None of the strains harbored any of the virulence factor-encoding genes of E. coli involved in acute enteric diseases, However, mannose-resistant adhesion to differentiated Caco-2 cells was found for 84.6% and 78.9% of the E. coli strains isolated from chronic and early recurrent lesions, respectively, compared with 33% of controls (P < 0.02), In addition, 21.8% of the strains induced a cytolytic effect by synthesis of an alpha-hemolysin. Conclusions: E. coli strains isolated from the ileal mucosa of patients with Crohn's disease adhere to differentiated intestinal cells and may disrupt the intestinal barrier by synthesizing an alpha-hemolysin.