Potential role of pulsed-high intensity focused ultrasound in gene therapy.
Potential role of pulsed-high intensity focused ultrasound in gene therapy.
复制标题
DOI：
10.2217/14796694.2.1.111
复制
发表时间：
2006-02-01
期刊：
影响因子：
--
通讯作者：
Li, King C P
中科院分区：
文献类型：
--
作者：
Frenkel, Victor;Li, King C P
随着对人类癌症生物学认识的加深，新的潜在的基因治疗策略正在被提出和评估。然而，安全高效的基因转移仍然是其在临床上实施的主要障碍。临床前研究表明，脉冲高强度聚焦超声(HIFU)照射可以与不同的给药模式(局部、血管内和全身)相结合，以改善基因和其他治疗剂的局部传递。使用图像引导，可以给出曝光，其中短能量脉冲在组织中主要产生机械/结构效应，而不是热效应。其结果是大分子的外渗和间质扩散都增加了，这是非破坏性的和可逆的。也可以添加超声造影剂，以增强声空化活性，从而增强声学造影剂。由于脉冲HIFU能够局部增加DNA的摄取和表达，因此有望进一步将基因治疗用于癌症和其他病理疾病的治疗。
As the understanding of human cancer biology increases, new potential strategies for gene therapy are being proposed and evaluated. However, safe and efficient gene transfer continues to be the major hurdle for its implementation in the clinic. Preclinical studies have shown how pulsed-high intensity focused ultrasound (HIFU) exposures can be combined with different modes of administration (local, intravascular and systemic) to improve local delivery of genes and other therapeutic agents. Using image guidance, exposures are given, where short pulses of energy create predominantly mechanical/structural effects in the tissues as opposed to thermal ones. The result is an increase in both extravasation and interstitial diffusion of macromolecules, which occur non-destructively and reversibly. Ultrasound contrast agents can also be added, which enhance acoustic cavitation activity and consequently sonoporation. By being able to locally increase the uptake and expression of DNA, pulsed-HIFU holds much promise to further the use and applications of gene therapy for treating cancer and other pathological conditions.