Elevated neuronal c-Fos-like immunoreactivity and messenger ribonucleic acid (mRNA) in genetically obese (ob/ob) mice.
Elevated neuronal c-Fos-like immunoreactivity and messenger ribonucleic acid (mRNA) in genetically obese (ob/ob) mice.
复制标题
遗传性肥胖 (ob/ob) 小鼠神经元 c-Fos 样免疫反应性和信使核糖核酸 (mRNA) 升高。
DOI：
10.1016/0006-8993(94)90281-x
复制
发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Romsos,DR
中科院分区：
文献类型：
--
作者：
Mistry,AM;Helferich,W;Romsos,DR
Adult genetically obese (ob/ob) mice display a number of metabolic alterations, the primary cause of which may be a defect in their central nervous system (CNS). The protein encoded by the protooncogene c-fos, c-Fos, functions as a nuclear transcription factor, and also serves as a marker of neuronal activity. The specific objectives of this study were (1) to use c-Fos immunohistochemistry to identify regions with altered neuronal activity in 6–7 week old male lean andob/obmice; (2) to examine c-fosrelative mRNA abundance by northern blot analysis in brains of these mice and compare it with that of neuropeptide Y (NPY), a peptide well known to alter feeding and (3) determine changes in c-Fos immunoreactivity and mRNA caused by food deprivation. Fos-like immunoreactivity (FLI) tended to be higher in ad libitum fedob/obmice than in lean controls in most brain regions examined. The most prominent and consistent differences were in the paraventricular nuclei (PVN) where the numbers of Fos-positive nuclei were approximately 3 hold higher inob/obmice. Food deprivation for 24 h increased FLI in the PVN in lean mice but did not further augment FLI in the PVN ofob/obmice. Arcuate nuclei of lean andob/obmice showed minimal FLI staining under ad libitum fed conditions. Food deprivation however, induced FLI in acurate nuclei of both lean andob/obmice. The abundance of c-fosmRNA in whole brain ofob/obmice averaged several fold higher than in leans under both fed and fasted conditions. There was no difference in whole brain NPY mRNA between lean andob/obmice. Prolonged stimulation of c-Fos in specific neurons ofob/obmice including the paraventricular nucleus probably has modulatory effects on certain genes which in turn account for long term adaptive changes of theob/obphenotype.
